Milli q gradient a 10 water purification system

All solutions were prepared with analytical reagent grade chemicals and ultra-pure water (18 MΩ cm) generated by purified distilled water with a Millipore Milli-Q Gradient A-10 water purification system (Bedford, MA, USA). All the plastic and glassware were cleaned by soaking in 2 M HNO₃ for 48 h, and rinsed five times with distilled water, and then five times with deionized water prior to use. The calibration standard solutions were freshly prepared by dilution of Optima Family Multi-Element Standard, Matrix per Volume: 2% HNO₃ and Multi-Element Calibration Standard 3, Matrix per Volume: 5% HNO₃ stock solution (PerkinElmer^®, Waltham, MA, USA). A DORM-2 (NRCC, Ottawa, ON, Canada) certified dogfish tissue was used as the calibration verification standard. Recoveries between 90.5 and 108% were accepted to validate the calibration.

TAC monohydrate >98% and hydroxypropyl cellulose (HPC, MW 100,000) were purchased from Sigma-Aldrich (St Louis, MO, USA). Deuterated TAC-¹³C₃D₂ (>85%) and Beagle dog plasma were obtained from Toronto Research Chemicals, Ontario, Canada, and BioChemed Services, Winchester, VA, USA, respectively. BioSustane™ SAIB and microcrystalline cellulose (MCC, Avicel^® PH-101) were obtained from Eastman Chemical Company (Kingsport, TN, USA) and FMC Corporation (Princeton, NJ, USA), respectively. Hydroxypropyl methylcellulose (HPMC, 50 cps), magnesium stearate (MGS), croscarmellose sodium (CCS), lactose monohydrate, orthophosphoric acid (OPA), methanol, ammonium acetate, zinc sulfate (ZnSO₄) and formic acid were purchased from Fisher Scientific (Asheville, NC, USA). Sodium lauryl sulfate (SLS) was purchased from VWR Chemicals, LLC (Fountain Parkway, OH, USA). All reagents were of analytical grade and used as received. In-house water (18 MΩcm, Millipore Milli-Q Gradient A-10 water purification system) was used in the study.

All solutions were prepared with analytical reagent grade chemicals and ultra-pure water (18 MΩ cm) generated by purified distilled water with a Millipore Milli-Q Gradient A-10 water purification system (Bedford, MA, USA). All the plastic and glassware were cleaned by soaking in 2 M HNO₃ for 48 h, and rinsed five times with distilled water, and then five times with deionized water prior to use. The calibration standard solutions were freshly prepared by dilution of Optima Family Multi-Element Standard, Matrix per Volume: 2% HNO₃, and Multi-Element Calibration Standard 3, Matrix per Volume: 5% HNO₃ stock solution (Perkin Elmer^®, Waltham, MA, USA).

Dithiotheritol (DTT), iodoacetamide (IA), ammonium bicarbonate (NH₄HCO₃) and hydrochloric acid (HCl, 37%) were obtained from Sigma–Aldrich (St. Louis, MO, USA). Formic acid (FA) and acetonitrile (ACN) were purchased from Merck (Darmstadt, Germany). All the reagents used were analytical or HPLC grade. Sequencing grade modified trypsin was from Shanghai Yaxin Biotechnology Co., Ltd (Shanghai, China). All chemical agents were prepared using ultrapure water and without further purification. Ultrapure water was obtained by a Milli-Q Gradient A10 water purification system (Millipore, Bedford, MA, USA) during all the experiments.

Ammonium bicarbonate (NH₄HCO₃), dithiothreitol (DTT), iodoacetamide (IA) and hydrochloric acid (HCl, 37%) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile (ACN) and formic acid (FA) were purchased from Merck (Darmstadt, Germany). All the reagents used were analytical or HPLC grade. Sequencing grade modified trypsin was from Shanghai Yaxin Biotechnology Co., Ltd (Shanghai, China). All chemical agents were prepared using ultrapure water and without further purification. Ultrapure water was obtained by a Milli-Q Gradient A10 water purification system (Millipore, Bedford, MA, USA) during all the experiments.