The crude toxin preparation is made by condensing the fermentation broth through Concentrator (N-1100 rotary evaporator, EYELA, Tokyo, Japan). Three liters fermentation broth was condensed into 100 ml that was used as crude toxins. 5 μl crude toxin preparation was injected on a cotton leaf (four inoculation sites per leaf). Then, the leaves transferred into growth chamber at 26°C for 3 days. The disease damage was evaluated by staining lesions with 0.4% trypan blue.
N 1100 rotary evaporator
Manufactured by Eyela
Sourced in Japan
The N-1100 rotary evaporator is a laboratory instrument designed for the efficient evaporation and concentration of solvents. It features a rotating flask that is immersed in a water bath, allowing for the controlled, gentle removal of volatile components from liquid samples.
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Lab products found in correlation
2 protocols using n 1100 rotary evaporator
1
Crude Toxin Extraction and Leaf Assay
2
Extraction of White Tea Soluble Nutrients
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The white tip silver needle (Fuding, Fujian, China) was chopped, ground, and dried at 60°C. Then, 10 g of white tea powder was accurately weighed, and 250 mL of 70% ethanol was added. The filtrate was extracted for 60 minutes in a water bath at 60°C, and then it was extracted again. The extracted filtrates were combined, and WTSNF was obtained by using an N-1100 rotary evaporator (Eyela, Tokyo, Japan). The WTSNF was then put into a low-temperature drying dish for later use.
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