Images captured on an UltraView Vox Spinning Disk Confocal Microscope (PerkinElmer, Waltham MA, USA) using Volocity 6.3.1 software (PerkinElmer) and Nikon PlanFluor 20× and Nikon Apo TIRF 60× objectives, or a BZ-X810 widefield fluorescence microscope (Keyence, Osaka, Japan). In-house software (https://bitbucket.org/pythoncardiacmodel/publicpythoncardiacmodel/src/master/ ) and Volocity used for image stitching. Analyses and particle counts performed in Volocity or Fiji/Imagej [85 (link)].
Apo tirf 60
Manufactured by Nikon
Sourced in Japan
The Apo TIRF 60 is a high-performance objective lens designed for Total Internal Reflection Fluorescence (TIRF) microscopy. It features an Apochromatic optical design for superior image quality and chromatic aberration correction.
Automatically generated - may contain errors
2 protocols using apo tirf 60
1
Confocal Microscopy Imaging and Analysis
2
Single-molecule Sequencing Imaging System
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Our system was constructed using commercially available Nikon Ti-E (TI-ND6-PFS, Nikon) equipped with the following; ×60 objective lens (Apo TIRF ×60, Nikon) with ×1.5 transfer, EMCCD-camera (ImagEM-1K-N TDI-qEM-CCD, Hamamatsu Photonics), excitation laser 640 nm (CUBE 640–100 C, COHERENT), motorized stage system (BIOS-206T, SIGMAKOKI) with our custom stage holder, a custom heater plate (Custom, Tokai hit) and a reagent exchanging system with eight syringe pumps (Cavro XLP6000 with 9-port valves and 1 ml syringe, TECAN); (Supplementary Fig. 12 ). A custom flow cell was placed on the stage and connected to the reagent exchange system via PEEK tubes (Supplementary Fig. 13 ). Sequencing reagents were introduced through the reagent exchange system. To perform sequencing reactions, buffer supplies and image acquisitions were regulated using custom software written in C#.
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