Primary antibody against gapdh

Half-fin anchovy (Setipinna taty) was purchased from a local aquatic market in Zhoushan, China. Pepsin (1200 U/g) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The assay kits of LDL-C, HDL-C, IL-6, and TNF-α were purchased from Jiancheng Bioengineering Institute (Nanjing, China). Primary antibodies against SOD1, SOD2, CAT, GPX2, Nrf2, and CML were purchased from Abclonal (Wuhan, China). Primary antibodies against Keap1, NQO-1, and HO-1 were bought from Abcam (Shanghai, China). A primary antibody against PEPT1 was purchased from Santa Cruz Biotechnology, Inc. (Beijing, China). A primary antibody against GAPDH was purchased from Sigma (St Louis, MO, USA). An HRP-labeled goat anti-rabbit/mouse IgG (PV-9000) universal two-step kit was obtained from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. China. Other chemicals of analytical grade were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

Recombinant human IL-32α (rIL-32α), Recombinant human IL-6 (rIL-6) and neutralization antibodies to human IL-6 were purchased from R&D systems (Minneapolis, MN, USA). Antibodies to human IL-32, PR3, and immunofluorescence antibody to IL-32 were purchased from Abcam (Cambridge, UK). Western blot antibodies to STAT3, pSTAT3 Ser-727, pSTAT3 Tyr-727, p38MAPK, pp38MAPK, p65 and pp65 were purchased from Cell Signaling Technology (Danvers, MA, USA). NF-κB inhibitor QNZ and STAT3 inhibitor BP-1-102 were purchased from Selleckchem (Houston, TX, USA). Primary antibody against GAPDH was purchased from Sigma-Aldrich. Horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse antibodies were purchased from Jackson Immuno research Laboratories (West Grove, PA, USA).

DETA NONOate(DETA), L-NAME, N-PLA, 1400W, and the Griess agents were purchased from Cayman Chemical (Ann Arbor, MI, USA). Chloroquine diphosphate salt, Akt1/2 kinase inhibitor, DDP, 1,4-Dithiothreitol, and thiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St Louis, MO, USA), rapamycin was purchased from LC Laboratories (Woburn, MA, USA). The primary antibody against GAPDH and β-actin was purchased from Sigma-Aldrich. Antibodies recognizing LC3B, p-AKT(Ser473), AKT, p-mTOR (Ser2448), mTOR, p-S6 (S235/236), S6, and p-AMPKα (Thr172) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against Beclin1, p62, NOS1, PTEN, and AMPK were obtained from Abcam (Cambridge, MA, USA). Atg16 L were purchased from MBL (Nagoya, Japan). Secondary antibody peroxidase conjugated goat anti-rabbit IgG (h+l) and peroxidase conjugated goat anti-mouse IgG (h+l) were purchased from fdbiotech (Guangzhou, China). Goat anti-rabbit Alexa Fluor 488and DAPI were purchased from Invitrogen (Carlsbad, CA, USA).