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2 protocols using actr5

1

Antibody-Mediated Protein Interactome Analysis

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Antibodies used include: GSDMD Rabbit polyclonal Ab (Novus Biologicals, NBP2-33422), GSDMD Rabbit monoclonal Ab (Abcam, Ab209845), FLAGĀ® M2 monoclonal Ab (Sigma, F3165), CARD8 C terminus Rabbit polyclonal Ab (Abcam, Ab24186), CARD8 N-terminal Rabbit polyclonal Ab (Abcam, Ab194585), GAPDH Rabbit monoclonal Ab (Cell Signaling Tech, 14C10), MTMR1 (Abcam, Ab 240569), D2HGDH (Abcam, Ab233516), ATF-3 (Cell Signaling Tech, D2Y5W), IGBP1 (Cell Signaling Tech, 5F6), SMAD9 (Abcam, Ab124094), ARF1 (Abcam, Ab58578), ACTR5 (Proteintech, 21505), AKAP8 (Abcam, Ab72196), GSTP1 (Cell Signaling Tech, 3F2), SET (Abcam, Ab1183), NLRP1B (Vance laboratory, 2A12), IRDye 800CW anti-rabbit (LICOR, 925-32211), IRDye 800CW anti-mouse (LI-COR, 925-32210), IRDye 680CW anti-rabbit (LI-COR, 925-68073), IRDye 680CW anti-mouse (LI-COR, 925-68072). Other reagents used include: Val-boroPro (VbP) (Okondo et al., 2017 (link)), Bortezomib (Bort; MilliporeSigma, 504314), MLN4924 (Cayman, 15217), bestatin methyl ester (Me-Bs; Sigma, 200485), sequencing grade modified trypsin (Promega, V5113), proteinase K (PROK; Invitrogen, 25530049), FuGENE HD (Promega, E2311), TMTsixplex Isobaric Label Reagents (ThermoFisher Scientific).
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2

Antibody-Mediated Protein Interactome Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used include: GSDMD Rabbit polyclonal Ab (Novus Biologicals, NBP2-33422), GSDMD Rabbit monoclonal Ab (Abcam, Ab209845), FLAGĀ® M2 monoclonal Ab (Sigma, F3165), CARD8 C terminus Rabbit polyclonal Ab (Abcam, Ab24186), CARD8 N-terminal Rabbit polyclonal Ab (Abcam, Ab194585), GAPDH Rabbit monoclonal Ab (Cell Signaling Tech, 14C10), MTMR1 (Abcam, Ab 240569), D2HGDH (Abcam, Ab233516), ATF-3 (Cell Signaling Tech, D2Y5W), IGBP1 (Cell Signaling Tech, 5F6), SMAD9 (Abcam, Ab124094), ARF1 (Abcam, Ab58578), ACTR5 (Proteintech, 21505), AKAP8 (Abcam, Ab72196), GSTP1 (Cell Signaling Tech, 3F2), SET (Abcam, Ab1183), NLRP1B (Vance laboratory, 2A12), IRDye 800CW anti-rabbit (LICOR, 925-32211), IRDye 800CW anti-mouse (LI-COR, 925-32210), IRDye 680CW anti-rabbit (LI-COR, 925-68073), IRDye 680CW anti-mouse (LI-COR, 925-68072). Other reagents used include: Val-boroPro (VbP) (Okondo et al., 2017 (link)), Bortezomib (Bort; MilliporeSigma, 504314), MLN4924 (Cayman, 15217), bestatin methyl ester (Me-Bs; Sigma, 200485), sequencing grade modified trypsin (Promega, V5113), proteinase K (PROK; Invitrogen, 25530049), FuGENE HD (Promega, E2311), TMTsixplex Isobaric Label Reagents (ThermoFisher Scientific).
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