Synergy fluorescence spectrophotometer

Manufactured by Agilent Technologies

The Synergy fluorescence spectrophotometer is a versatile instrument designed for accurate and sensitive fluorescence measurements. It features advanced optics and a high-performance detector to deliver reliable data across a wide range of applications.

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Fluorescence spectrophotometer (60 citations) Synergy spectrophotometer (18 citations) SynergyTM H1 Hybrid Multi-Mode fluorescence spectrophotometer (2 citations) Synergy H1 Fluorescence Spectrophotometer (2 citations)

3 protocols using synergy fluorescence spectrophotometer

Comparative Photobleaching of ICG Formulations

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An aqueous solution of free ICG, ICG encapsulated PEGylated P(R^DGD) and the non-PEGylated P(R^DGD) NPs in water were prepared to give similar fluorescence intensity. The fluorescence intensities were measured with λ_ex set at 780 nm and λ_em set at 800 nm for ICG. Each of the samples was illuminated continuously with a xenon lamp, and the fluorescence intensity was measured over a period of 30 min by a Synergy fluorescence spectrophotometer (Agilent Technologies Inc.). Intensity values were normalized for comparison.

Hadad E., Rudnick-Glick S., Grinberg I., Yehuda R, & Margel S. (2020). Engineering of NIR fluorescent PEGylated poly(RGD) proteinoid polymers and nanoparticles for drug delivery applications in chicken embryo and mouse models. RSC Advances, 10(57), 34364-34372.

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Quantifying Cy3 Fluorescence Intensity

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An aqueous solution of free Cy3 was prepared in order to provide the same fluorescence intensity as the corresponding Cy3-conjugated NPs aqueous dispersion. The fluorescence intensities of the free and conjugated Cy3 were measured at excitation and emission wavelengths λex and λem set to 540 and 650 nm, respectively. Samples were illuminated continuously with a xenon lamp, and the fluorescence intensity of Cy3 was measured over 30 min by a Synergy fluorescence spectrophotometer (Agilent Technologies Inc.) and normalized to allow comparison.

Sasson E., Pinhasi R.V., Margel S, & Klipcan L. (2020). Engineering and use of proteinoid polymers and nanocapsules containing agrochemicals. Scientific Reports, 10, 9171.

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Fluorescence Stability of Dox and Dox/P(RGD) NCs

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Aqueous solutions/dispersions of Dox and Dox/P(RGD) NCs were prepared to give similar fluorescence intensity. Intensities were measured with λex set at 490 nm and λem of 590 nm. Each sample was illuminated continuously with a xenon lamp, and the fluorescence intensity was measured over a period of 5 min by a Synergy fluorescence spectrophotometer (Agilent Technologies Inc.). Intensity values were normalized for comparison.

Hadad E., Rudnick-Glick S., Itzhaki E., Avivi M.Y., Grinberg I., Elias Y, & Margel S. (2020). Engineering of Doxorubicin-Encapsulating and TRAIL-Conjugated Poly(RGD) Proteinoid Nanocapsules for Drug Delivery Applications. Polymers, 12(12), 2996.

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