Ultimate 3000 ultra-high performance liquid chromatography system (Thermo Fisher, CA, USA) was connected to a Q-Exactive Orbitrap tandem mass spectrometer (Thermo Fisher, CA, USA) via an ESI source. Oil-Free Air Compressors (AC-1Y) was purchased from Beijing Purkinje General Instrument Co., Ltd. (Beijing, China). High-speed refrigerated centrifuge was acquired from Heraeus Holding GmbH (GER). MX-F vortex Mixers was provided by Wuhan Servicebio Technology Co., Ltd. (Wuhan, China). Both CNC ultrasonic cleaner (Q-250D, Kun Shan Ultrasonic Instruments Co., Ltd.) and 1/10000 electronic scale (XS205DU, Mettler Toledo International Trading (Shanghai) Co., Ltd.) were also used in quantitative analysis.
Q exactive orbitrap tandem mass spectrometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Q-Exactive Orbitrap tandem mass spectrometer is a high-resolution, high-accuracy mass analyzer used for the identification and quantification of molecular compounds. It combines a quadrupole mass filter and an Orbitrap mass analyzer to provide precise mass measurement and sensitive detection of a wide range of analytes.
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Lab products found in correlation
5 protocols using q exactive orbitrap tandem mass spectrometer
1
Quantitative Analysis by UHPLC-MS
2
Phytochemical Analysis of SMY Decoction
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The SMY decoction was analyzed by ultrahigh performance liquid chromatography quadrupole Orbitrap high resolution mass spectrometry (UPLC-Q-Orbitrap HRMS) (Thermo Fisher Scientific, USA) with an Accucore C chromatographic column (3 mm × 100 mm, 2.6 μm, Thermo Fisher Scientific, USA). The mobile phase consisted of (A) 100% water with 0.1% formic acid and (B) 100% acetonitrile. Gradient elution was conducted using 5%-15% (0–10 min), 15% ~ 18% (10 ~ 18 min), 18% ~ 22% (18 ~ 20 min), 22% ~ 25% (20 ~ 35 min), 25% ~ 30% (35 ~ 45 min), and 30% ~ 40% acetonitrile (45 ~ 60 min). The flow rate was 0.3 mL/min. High resolution mass detection was performed on a Q-Exactive Orbitrap tandem mass spectrometer (Thermo Fisher Scientific, USA) equipped with a heated electrospray ionization source (ESI) operated in the positive and negative ion mode. The ESI source parameters were set as follows: ion spray voltage at 3 kV (±), probe heater temperature at 350 ℃, sheath gas flow rate at 35 arbitrary units, the auxiliary gas flow rate at 10 arbitrary units, and the ion transfer tube temperature at 320 ℃. Full mass spectra were obtained from m/z 100 to 1500 with a resolution of 70,000, while data dependent MS2 (dd-MS2) spectra were acquired at a resolution of 35,000 with the ramp collision energy at 20, 40, and 60 eV.
3
Supercritical Fluid Extraction and UPLC-MS Analysis
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SFE was operated
with an ASI Supercritical Fluid Extraction system (SPE-ed SFE-2) (Applied
Separations, Inc., Allentown, PA) equipped with a high-pressure CO2 ASF-100 pump. Hk-04b swing crusher was produced from Guangzhou
Xuyang Machinery Equipment Co. Ltd., and the ME 104 electronic analytical
balance was provided by Mettler Toledo Instrument Co. Ltd. The UPLC
system mainly consisted of an Ultimate 3000 series ultra-high-performance
liquid chromatograph (ThermoFisher Scientific) equipped with an online
degasser, a quaternary pump, an autosampler, and a column temperature
compartment; it was connected to a Q-Exactive Orbitrap tandem mass
spectrometer (ThermoFisher Scientific) via an electrospray ionization
interface. Besides, the ZORBAX C18 column (2.1 mm ×
50 mm, 1.8 μm) was offered by Agilent Technologies.
with an ASI Supercritical Fluid Extraction system (SPE-ed SFE-2) (Applied
Separations, Inc., Allentown, PA) equipped with a high-pressure CO2 ASF-100 pump. Hk-04b swing crusher was produced from Guangzhou
Xuyang Machinery Equipment Co. Ltd., and the ME 104 electronic analytical
balance was provided by Mettler Toledo Instrument Co. Ltd. The UPLC
system mainly consisted of an Ultimate 3000 series ultra-high-performance
liquid chromatograph (ThermoFisher Scientific) equipped with an online
degasser, a quaternary pump, an autosampler, and a column temperature
compartment; it was connected to a Q-Exactive Orbitrap tandem mass
spectrometer (ThermoFisher Scientific) via an electrospray ionization
interface. Besides, the ZORBAX C18 column (2.1 mm ×
50 mm, 1.8 μm) was offered by Agilent Technologies.
4
Analytical Instrumentation for Compound Characterization
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The
SB25-12DTD ultrasonic cleaners were obtained from Qunshan Machinery
Equipment (China), and the Hk-04b swing crusher and the ME-104 electronic
analytical balance were purchased from Xuyang Machinery Equipment
(China) and Mettler Toledo (China), respectively. The XHRE-2000C rotary
evaporator connected with a XHDL-200 low-temperature circulating pump
was obtained from Shanghai Xiaohan Industrial Development Company
(China). The ZORBAX Rclipse Plus C18 column (2.1 mm ×
50 mm, 1.8 μm) was purchased from Agilent Technologies (USA).
The UPLC-Q-Exactive Orbitrap/MS system equipped with a Dionex Ultimate
3000 UPLC system (Thermo Scientific, USA) consisted of an autosampler,
an online degasser, a quaternary pump, and a column temperature compartment,
and it was combined with a Q Exactive Orbitrap tandem mass spectrometer
(Thermo Scientific, USA) by means of an electrospray ionization interface.
SB25-12DTD ultrasonic cleaners were obtained from Qunshan Machinery
Equipment (China), and the Hk-04b swing crusher and the ME-104 electronic
analytical balance were purchased from Xuyang Machinery Equipment
(China) and Mettler Toledo (China), respectively. The XHRE-2000C rotary
evaporator connected with a XHDL-200 low-temperature circulating pump
was obtained from Shanghai Xiaohan Industrial Development Company
(China). The ZORBAX Rclipse Plus C18 column (2.1 mm ×
50 mm, 1.8 μm) was purchased from Agilent Technologies (USA).
The UPLC-Q-Exactive Orbitrap/MS system equipped with a Dionex Ultimate
3000 UPLC system (Thermo Scientific, USA) consisted of an autosampler,
an online degasser, a quaternary pump, and a column temperature compartment,
and it was combined with a Q Exactive Orbitrap tandem mass spectrometer
(Thermo Scientific, USA) by means of an electrospray ionization interface.
5
Ultra-high-performance LC-MS analysis
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The ultra-high-performance
LC was carried out on an Dionex UltiMate 3000 UHPLC system (Thermo
Scientific, USA) equipped with an online degasser, a quaternary pump,
an autosampler, and a column temperature compartment, which was connected
to a Q-Exactive Orbitrap tandem mass spectrometer (Thermo Scientific,
USA) via an electrospray ionization interface and high-resolution
MS detection was performed in the positive ion mode, and the data
were acquired in full scan mode. A ZORBAX Eclipse Plus C18 (2.1 mm × 50 mm, 1.8 μm) was utilized at 40 °C and
eluted with a gradient solvent from A/B (90:10) to A/B (10:90) at
a flow rate of 0.50 mL/min, where A is 0.1% (v/v) formic acid solution
and B is 0.1% (v/v) formic acid solution of acetonitrile. The linear
gradient elution was as follows: 0–2 min, 10–25% B;
2–4 min, 25–25% B; 4–10 min, 25–50% B;
10–14 min, 50–50% B; 14–18 min, 50–85%
B; and 18–19 min, 85–90% B.
LC was carried out on an Dionex UltiMate 3000 UHPLC system (Thermo
Scientific, USA) equipped with an online degasser, a quaternary pump,
an autosampler, and a column temperature compartment, which was connected
to a Q-Exactive Orbitrap tandem mass spectrometer (Thermo Scientific,
USA) via an electrospray ionization interface and high-resolution
MS detection was performed in the positive ion mode, and the data
were acquired in full scan mode. A ZORBAX Eclipse Plus C18 (2.1 mm × 50 mm, 1.8 μm) was utilized at 40 °C and
eluted with a gradient solvent from A/B (90:10) to A/B (10:90) at
a flow rate of 0.50 mL/min, where A is 0.1% (v/v) formic acid solution
and B is 0.1% (v/v) formic acid solution of acetonitrile. The linear
gradient elution was as follows: 0–2 min, 10–25% B;
2–4 min, 25–25% B; 4–10 min, 25–50% B;
10–14 min, 50–50% B; 14–18 min, 50–85%
B; and 18–19 min, 85–90% B.
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