Ft ir spectrum rx 1

Fourier transform infrared spectroscopy (FT-IR) spectra of the SPE, PC, and SPEPC, were taken (Perkin Elmer FT-IR-Spectrum RX 1) in the transmission mode with the wave number region 4000–500 cm⁻¹.

A FT-IR Spectrum RX 1 (PerkinElmer) was employed to acquire the Fourier Transform−Infrared spectrum in the frequency scale of 400–4000 cm⁻¹. For analysis, lyophilised polysaccharide (P) ground with KBr (1:20) was utilised for pellet assembling. Ultraviolet-visible and fluorescence spectra were acquired on Shimadzu UV-2450 and Hitachi F−7000 spectrophotometer, respectively. The ¹H NMR spectrum of fraction P was obtained via a Varian 400MR spectrometer at 25 °C. The hydroxylic protons of polysaccharide sample (10 mg) were replaced with deuterium using 99.9% D₂O (Sigma Aldrich) and this process repeated thrice to diminish the signal residual proton. This deuterium exchanged sample was dissolved in 500 μL D₂O for subsequent proton NMR measurement. The electrospray ionization mass spectrum of per-O-acetylated oligomers (O) was acquired by means of a QTOF 60 Micro YA 263 mass spectrometer.