Uterine tubular gland cells were lysed in lysis buffer (BestBio) and the total protein concentration was quantified using a BCA assay (BestBio) according to the manufacturer’s protocol. Immunoblots were performed using primary and secondary antibodies such as anti-ATP2B4 (PMCA4) (1:1000, Abcam) and goat anti-mouse IgG (Zen-Bio, Chengdu, China) respectively. Western blotting was performed as described previously [71 (link)].
Bca assay
Manufactured by BestBio
The BCA assay is a colorimetric detection method used for the quantitative determination of total protein concentration in a sample. It relies on the reduction of copper ions by proteins in an alkaline medium, resulting in a purple-colored complex that can be measured spectrophotometrically.
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Lab products found in correlation
3 protocols using bca assay
1
Quantification and Immunoblotting of PMCA4
2
Quantitative Protein Analysis in Uterine Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Uterine tubular gland cells were lysed in lysis buffer (BestBio) and the total protein concentration was quantified using a BCA assay (BestBio) according to the manufacturer's protocol.
Immunoblots were performed using prescribed primary and secondary antibodies such as anti-ATP2B4 (PMCA4) (1:1000, Abcam) and goat anti-mouse IgG (Zen-Bio, Chengdu, China) respectively. Western blot procedures were conducted as previously described [67] .
Immunoblots were performed using prescribed primary and secondary antibodies such as anti-ATP2B4 (PMCA4) (1:1000, Abcam) and goat anti-mouse IgG (Zen-Bio, Chengdu, China) respectively. Western blot procedures were conducted as previously described [67] .
3
Quantitative Protein Analysis in Uterine Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Uterine tubular gland cells were lysed in lysis buffer (BestBio) and the total protein concentration was quantified using a BCA assay (BestBio) according to the manufacturer's protocol.
Immunoblots were performed using prescribed primary and secondary antibodies such as anti-ATP2B4 (PMCA4) (1:1000, Abcam) and goat anti-mouse IgG (Zen-Bio, Chengdu, China) respectively. Western blot procedures were conducted as previously described [67] .
Immunoblots were performed using prescribed primary and secondary antibodies such as anti-ATP2B4 (PMCA4) (1:1000, Abcam) and goat anti-mouse IgG (Zen-Bio, Chengdu, China) respectively. Western blot procedures were conducted as previously described [67] .
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