Sc 71557

Manufactured by Santa Cruz Biotechnology

Sc-71557 is a laboratory product offered by Santa Cruz Biotechnology. It is a sterile, cell culture-tested reagent. The core function of Sc-71557 is to serve as a research tool for scientific investigations.

Automatically generated - may contain errors

Lab products found in correlation

Gtx128354, by GeneTex (2 mentions) Rabbit anti pan cadherin, by Merck Group (2 mentions) Sc 6556, by Santa Cruz Biotechnology (2 mentions) Gfp 1010, by Aves Labs (2 mentions) Lsm 710, by Zeiss (2 mentions) Goat anti hnf4a, by Santa Cruz Biotechnology (1 mentions) Ab96415, by Abcam (1 mentions) Ab76997, by Abcam (1 mentions) Ab181598, by Abcam (1 mentions)

2 protocols using sc 71557

Whole-mount in situ hybridization and immunofluorescence

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole-mount in situ hybridization was performed as previously described using the sox9b probe (30 (link)). IF on whole-mount animals or cryosections was performed as previously described (58 (link)) (59 (link)). Antibodies used include rabbit anti-Prox1a (1:100, GTX128354; GeneTex), goat anti-Hnf4a (1:50, sc6556; Santa Cruz), mouse anti-Alcama (1:20, zn-8; DSHB), rabbit anti-pan-Cadherin (1:5,000; Sigma), mouse anti-Mdr1 (1:300, sc-71557; Santa Cruz), mouse anti-Anxa4 (1:100, ab71286, aka 2F11; Abcam) (8 (link)), chicken anti-GFP (1:300, GFP1010; Aves Labs), goat anti-mCherry (1:500, LS-C204207; LSBio), rabbit anti-Sox9b (1:100; gift from Mizuki Azuma at the University of Kansas), rabbit anti-Cytokeratin 19 (CK19) (1:100; ET1601-6; HUABIO), and rabbit anti-SOX9 (1:100; ET1611-56; HUABIO).

Zhao C., Matalonga J., Lancman J.J., Liu L., Xiao C., Kumar S., Gates K.P., He J., Graves A., Huisken J., Azuma M., Lu Z., Chen C., Ding B.S, & Dong P.D. (2022). Regenerative failure of intrahepatic biliary cells in Alagille syndrome rescued by elevated Jagged/Notch/Sox9 signaling. Proceedings of the National Academy of Sciences of the United States of America, 119(50), e2201097119.

+ Open protocol

+ Expand

Multimarker Fluorescent Imaging of Liver

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole-mount fluorescent in situ hybridization was performed as previously described using the fgf10 probe(16 (link)). Whole mount IF staining was performed as previously described (16 (link)). Antibodies used include rabbit anti-Prox1a (1:100, GTX128354, GeneTex), goat anti-Hnf4a (1:50, sc6556, Santa Cruz), mouse anti-Alcama (1:20, zn-8, DSHB) and mouse anti-Islet1/2 (1:20, 39.4D5, DSHB), rabbit anti-pan-Cadherin (1:5000; Sigma), mouse anti-Anxa4 (1:100, ab71286, aka 2F11, Abcam)(15 (link)), rabbit anti-Bhmt (1:200, ab96415, Abcam), mouse anti-Mdr1(1:200, sc-71557, Santa Cruz), chicken anti-GFP (1:300, GFP1010, Aves Labs), goat anti-mCherry (1:500, LS-C204207, LSBio), rabbit anti-Cytokeratin7 (1:200, ab181598, Abcam), mouse anti-Sox9 (1:200, ab76997, Abcam). Imaging was performed on a Zeiss LSM710 running Zen 2010 (Black) and images were processed with Adobe Photoshop.

Zhao C., Lancman J.J., Yang Y., Gates K.P., Cao D., Barske L., Matalonga J., Pan X., He J., Graves A., Huisken J., Chen C, & Dong P.D. (2021). Intrahepatic cholangiocyte regeneration from an Fgf-dependent extrahepatic progenitor niche in a zebrafish model of Alagille Syndrome. Hepatology (Baltimore, Md.), 75(3), 567-583.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!