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Peroxidase conjugated secondary antibodies for western blotting

Manufactured by Merck Group

Peroxidase conjugated secondary antibodies for western blotting. These antibodies are designed to detect and bind to primary antibodies used in western blot analysis, allowing for visualization of target proteins.

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2 protocols using peroxidase conjugated secondary antibodies for western blotting

1

Antibodies for Protein Analysis

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Rabbit polyclonal DPY30, RBBP5, ASH2L and BIG1 antibodies were from Bethyl Laboratories. Rabbit polyclonal anti-GFP (6556) and mouse monoclonal anti-M6PR (ab2733) were from Abcam. Mouse monoclonal anti-Snx1 was obtained from BD Transduction Laboratories. Mouse monoclonal anti-LC3 was obtained from MBL (clone 4E12, catalogue number M152-3). Rabbit polyclonal anti-spastin 86-340 and CD8 antibodies were produced as previously described (Seaman, 2004 (link); Connell et al., 2009 (link)). Mouse monoclonal anti-LAMP1 (H4A3) was obtained from Santa Cruz Biotechnology. Rabbit polyclonal anti-GAPDH (2118) was from Cell Signalling Technology. Alexa Fluor® 488 and 568 labelled secondary antibodies for immunofluorescence were obtained from Molecular Probes. Peroxidase conjugated secondary antibodies for western blotting were obtained from Sigma.
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2

Antibodies for Spastin and Microtubule Proteins

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Rabbit polyclonal anti-spastin (86-340) was produced as previously described [23 (link)]. Mouse monoclonal anti-spastin (6C6) and mouse monoclonal anti-Acetylated tubulin (6-11B-1) were obtained from Sigma-Aldrich. Mouse monoclonal anti-myc (4A6) was obtained from Millipore. Rabbit polyclonal anti-myc tag (ab9106), rabbit anti-GFP (ab6556) and rat anti-alpha-tubulin-tyrosinated clone YL12/2 (ab6160) were obtained from Abcam. Rat anti-RFP (5F8) was obtained from Chromotek. Goat anti-BMPR2 (EB08820) and goat anti-Kinesin-1/KIF5 (EB05492) were obtained from Everest Biotech. Rabbit polyclonal anti-ZFYVE27 (12680-1AP) and anti-IST1 (51002-1-AP) were obtained from Proteintech. Mouse monoclonal anti-TfnR (13-6800) was obtained from Invitrogen. Rabbit polyclonal anti-GAPDH (2118) was obtained from Cell Signaling Technology. Rabbit polyclonal anti-CHMP1B was produced as previously described [15 (link)]. Peroxidase conjugated secondary antibodies for western blotting were obtained from Sigma-Aldrich. Alexafluor-labelled secondary antibodies for immunofluorescence were obtained from Molecular Probes.
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