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αpaf1

Manufactured by Fortis Life Sciences
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αPAF1 is a laboratory equipment product that functions as a polyclonal antibody. It is designed to detect and bind to the PAF1 protein, which is a component of the RNA polymerase II complex involved in transcriptional regulation.

Automatically generated - may contain errors

Lab products found in correlation

α gapdh, by Merck Group (2 mentions) α β actin, by Merck Group (2 mentions) αcdc73, by Fortis Life Sciences (2 mentions) αprmt5, by Merck Group (2 mentions) αsym10, by Merck Group (2 mentions) αh2bub, by Merck Group (2 mentions) αh3k4me3, by Abcam (2 mentions) αh3k79me2, by Abcam (2 mentions) αh4r3me2s, by Abcam (2 mentions) αr me2s, by Cell Signaling Technology (2 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Chemidoc xr, by Bio-Rad (2 mentions)

2 protocols using αpaf1

1

Western Blot Analysis of Histone Modifications

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blots were performed with whole cell lysates or acid extracted histones and probed with the following antibodies: αCDC73, αPAF1 (Bethyl, Montgomery, TX), αPRMT5, αSYM10, αGAPDH, αH2BUb (Millipore, Billerica, MA), αH3, αH3K4me3, αH3K79me2, αH4R3me2s (Abcam,Cambridge, UK), αR-Me2s (Cell Signaling, Danvers, MA), α-βActin (Sigma). Bands were detected using SuperSignal West Pico Chemiluminescent substrate (Thermo-Fisher Scientific, Waltham, MA) and imaged on a ChemiDocXRS (Bio-Rad, Hercules, CA).
Serio J., Ropa J., Chen W., Mysliwski M., Saha N., Chen L., Wang J., Miao H., Cierpicki T., Grembecka J, & Muntean A.G. (2017). The PAF Complex Regulation of Prmt5 Facilitates the Progression and Maintenance of MLL-Fusion Leukemia. Oncogene, 37(4), 450-460.
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2

Western Blot Analysis of Histone Modifications

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blots were performed with whole cell lysates or acid extracted histones and probed with the following antibodies: αCDC73, αPAF1 (Bethyl, Montgomery, TX), αPRMT5, αSYM10, αGAPDH, αH2BUb (Millipore, Billerica, MA), αH3, αH3K4me3, αH3K79me2, αH4R3me2s (Abcam,Cambridge, UK), αR-Me2s (Cell Signaling, Danvers, MA), α-βActin (Sigma). Bands were detected using SuperSignal West Pico Chemiluminescent substrate (Thermo-Fisher Scientific, Waltham, MA) and imaged on a ChemiDocXRS (Bio-Rad, Hercules, CA).
Serio J., Ropa J., Chen W., Mysliwski M., Saha N., Chen L., Wang J., Miao H., Cierpicki T., Grembecka J, & Muntean A.G. (2017). The PAF Complex Regulation of Prmt5 Facilitates the Progression and Maintenance of MLL-Fusion Leukemia. Oncogene, 37(4), 450-460.
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