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Rat albumin elisa kit

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The Rat Albumin ELISA kit is a quantitative assay designed to measure the concentration of albumin in rat serum or plasma samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the target analyte.

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Lab products found in correlation

Accu chek performa, by Roche (1 mentions) Bun colorimetric detection kit, by Thermo Fisher Scientific (1 mentions) Creatinine assay kit, by Abcam (1 mentions) Hd s10, by Data Sciences International (1 mentions) Rat bnp 45 elisa kit, by Abcam (1 mentions) Cobas 6800, by Roche (1 mentions) Urea assay kit, by Abcam (1 mentions) Ab65340, by Abcam (1 mentions) Ab223858, by Abcam (1 mentions) Rat urinary protein assay kit, by Chondrex (1 mentions)

11 protocols using rat albumin elisa kit

1

Quantification of Urinary Albumin and Progesterone

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the blood glucose level was determined by glucometer (Accu-Chek Performa, Roche Diagnostics). Urine samples were centrifuged at 4 °C and 2000 rpm for 10 min. The urinary albumin and creatinine concentrations in the supernatant were measured using Albumin Rat ELISA kit (Abcam, UK) and creatinine ELISA kit (Cusabio, China) according to the manufacturer’s protocols. The urinary albumin to creatinine ratio (UACR) was calculated based on the albuminuria and urine creatinine level (albumin mg/creatinine g) [16 (link)]. Serum progesterone and estradiol levels were measured by commercially available ELISA kits (Biocheck, Inc. Foster City CA, USA and Cusabio, wuhan, China, respectively), according to the manufacturer’s protocols.
Al-Trad B., Ashankyty I.M, & Alaraj M. (2015). Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats. Diabetology & Metabolic Syndrome, 7, 97.
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2

Urine and Plasma Biomarker Quantification

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Urine albumin was measured with the Albumin rat ELISA kit (Abcam, Cambridge, UK). Urine and plasma creatinine were measured with the Creatinine assay kit (Abcam). Blood urea nitrogen (BUN) was evaluated with the BUN colorimetric detection kit (Thermo Fisher Scientific, Waltham, MA, USA).
Dal Monte M., Cammalleri M., Pecci V., Carmosino M., Procino G., Pini A., De Rosa M., Pavone V., Svelto M, & Bagnoli P. (2018). Inhibiting the urokinase‐type plasminogen activator receptor system recovers STZ‐induced diabetic nephropathy. Journal of Cellular and Molecular Medicine, 23(2), 1034-1049.
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3

Evaluating Nephroprotective Effects of ZnONPs

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After 7 weeks of ZnONPs treatment, rat urine collection was performed using individual metabolic cages for 24 h. Urinary albumin concentration was measured using the Albumin Rat ELISA kit (cat no. ab235642, Abcam, UK) according to manufacturer instructions. Using commercially available kits, blood glucose and blood urea nitrogen levels (BUN) were measured (ARCOMEX, Jordan). The rat kidney weight (mg) to body weight (g) ratio was calculated to obtain the kidney weight index (KWI).
Alomari G., Al‐Trad B., Hamdan S., Aljabali A.A., Al Zoubi M.S., Al‐Batanyeh K., Qar J., Eaton G.J., Alkaraki A.K., Alshaer W., Haifawi S., Jemon K., Chellappan D.K., Dua K, & Tambuwala M.M. (2021). Alleviation of diabetic nephropathy by zinc oxide nanoparticles in streptozotocin‐induced type 1 diabetes in rats. IET Nanobiotechnology, 15(5), 473-483.
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4

Renal Function Assessment Post-Ablation

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Three and six weeks after renal ablation, the rats were placed in metabolic cages (Nalgene, Rochester, NY, USA) to collect 24-h urine. Urine samples were centrifuged at 5000× g for 15 min to remove debris, and the supernatant was analyzed. The variables measured were creatinine and blood urea nitrogen (BUN) (IL 300 plus, Clinical Chemistry Analyzer, Instrumentation Laboratory, Bedford, MA, USA), microalbuminuria (Albumin Rat ELISA Kit, Abcam Cambridge, MA, USA), and urinary N-acetyl-β-d-glucosaminidase (NAG) activity (spectrophotometric assay).
García Trejo E.M., Arellano Buendía A.S., Sánchez Reyes O., García Arroyo F.E., Arguello García R., Loredo Mendoza M.L., Tapia E., Sánchez Lozada L.G, & Osorio Alonso H. (2017). The Beneficial Effects of Allicin in Chronic Kidney Disease Are Comparable to Losartan. International Journal of Molecular Sciences, 18(9), 1980.
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5

Effects of Arhgef11 Deletion on Salt-Induced Albuminuria

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At 4 weeks of age, age-matched male SS-WT (n=9) and SS-Arhgef11−/− (n=10) were weaned onto a low-salt diet (0.3% NaCl). Twenty-four-hour urine collections were done at 6 weeks of age on low salt and then each group was placed on high salt (2.0% NaCl) for the remainder of the study. Albuminuria was measured using a rat albumin ELISA kit (Abcam, ab108789). At week 7, a subset of animals from each group (n=8) were implanted with telemetry transmitters (model HD-S10, Data Sciences International) as previously described.13 (link),16 (link) After 1 week of recovery, blood pressure was measured for 24 hours one day each week from weeks 8 to 15, followed by 24-hour urine collections were made for the determination of proteinuria and albuminuria. Euthanasia was performed after week 15 blood pressure, and urine collections were complete. Organ weights, serum, and kidney samples for histological examination were collected.
Johnson A.C., Wu W., Attipoe E.M., Sasser J.M., Taylor E.B., Showmaker K.C., Kyle P.B., Lindsey M.L, & Garrett M.R. (2020). Loss of Arhgef11 in the Dahl Salt-Sensitive Rat Protects Against Hypertension-Induced Renal Injury. Hypertension (Dallas, Tex. : 1979), 75(4), 1012-1024.
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6

Metabolic Biomarkers in Empagliflozin Treatment

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EDTA was added to blood samples followed by centrifugation (4,500 rpm) for 20 min at 4 ℃, then plasma was collected and stored at -20 ℃ until analysis. Urine samples were centrifuged (12,000 rpm) for 10 min at 4 ℃. The supernatant was frozen in liquid nitrogen until analysis. Levels of plasma creatinine, urea, glucose, and insulin as well as urinary creatinine, and total protein were detected using an automatic biochemistry analyzing system (Roche Cobas 6800, Roche Ltd, Switzerland). Levels of plasma BNP45 and urinary albumin were determined quantitatively using Rat BNP 45 ELISA Kit (Abcam, Cat#ab108816) and Rat Albumin ELISA Kit (Abcam, Cat#ab235642). The glomerular filtration rate-to-body weight ratio (GFR), albumin-to-creatinine ratio (ACR) were calculated. At a dose of 1 mg/day of empagliflozin urinary sodium and potassium excretion are not affected (data not shown).
Xiong Y., Delic D., Zeng S., Chen X., Chu C., Hasan A.A., Krämer B.K., Klein T., Yin L, & Hocher B. (2022). Regulation of SARS CoV-2 host factors in the kidney and heart in rats with 5/6 nephrectomy—effects of salt, ARB, DPP4 inhibitor and SGLT2 blocker. BMC Nephrology, 23, 117.
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7

Assessing Kidney Function via Urine Albumin

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Urine albumin levels was measured as a proxy for kidney function assessment using Rat Albumin ELISA kit according to manufacturer’s instruction (Abcam, ab108789).
Jahan F., Vasam G., Cariaco Y., Nik-Akhtar A., Green A., Menzies K.J, & Bainbridge S.A. (2023). A comparison of rat models that best mimic immune-driven preeclampsia in humans. Frontiers in Endocrinology, 14, 1219205.
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8

Quantify Albumin and Urea Secretion

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Albumin and urea synthesis were quantified in spheroid media supernatant samples using Rat Albumin ELISA Kit (Abcam) and Urea Assay kit (Abcam) following the manufacturer's protocol. Supernatant samples were collected 4 days after media changes over the 21-day culture period. Data was normalised to account for cell number variation.
Kyffin J.A., Sharma P., Leedale J., Colley H.E., Murdoch C., Harding A.L., Mistry P, & Webb S.D. (2019). Characterisation of a functional rat hepatocyte spheroid model. Toxicology in Vitro, 55, 160-172.
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9

Serum ALT and Albumin Quantification

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The serum ALT and albumin concentrations in the rats were measured using a Rat Alanine Aminotransferase ELISA kit (cat. no. ab285264, Abcam) and Rat Albumin ELISA kit (cat. no. ab108789, Abcam), respectively. All samples were processed and assayed according to the manufacturer's protocol.
Murata K., Kaji K., Nishimura N., Enomoto M., Fujimoto Y., Takeda S., Tsuji Y., Fujinaga Y., Takaya H., Kawaratani H., Namisaki T., Akahane T, & Yoshiji H. (2022). Rifaximin enhances the L-carnitine-mediated preventive effects on skeletal muscle atrophy in cirrhotic rats by modulating the gut-liver-muscle axis. International Journal of Molecular Medicine, 50(2), 101.
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10

Evaluating Kidney Function Metrics in Rats

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All rats were housed in metabolic cages for 24 h (Pestel et al.) for blood and urine collection. After urine collection, blood samples were obtained from the lateral tail veins. Serum and urine creatinine levels were evaluated using a creatinine assay kit (ab65340; Abcam Plc, Cambridge, U.K.). The estimated glomerular filtration rate (eGFR) was obtained from creatinine clearance measurement in ml/min as follows: eGFR = (Ucr × V)/Pcr, Ucr = creatinine concentration in urine (mg/dL), V = urine flow rate (mL/min), and Pcr = creatinine concentration in plasma or serum (mg/dL)59 .
The albuminuria levels were evaluated using a rat albumin ELISA Kit (ab108789; Abcam Plc, Cambridge, U.K.). The albuminuria results are expressed in ng/mL. Thereafter, urine creatinine and urine albumin levels were calculated as the urinary albumin-to-creatinine ratio to indicate the level of kidney damage.
Iampanichakul M., Poasakate A., Potue P., Rattanakanokchai S., Maneesai P., Prachaney P., Settheetham-Ishida W, & Pakdeechote P. (2022). Nobiletin resolves left ventricular and renal changes in 2K-1C hypertensive rats. Scientific Reports, 12, 9289.
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