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Collagenase 4 digestion buffer

Manufactured by MP Biomedicals
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Collagenase IV digestion buffer is a solution used in the process of tissue dissociation and cell isolation. It contains the enzyme collagenase IV, which is used to break down the collagen found in the extracellular matrix of tissues.

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Lab products found in correlation

Taqman reverse transcriptase kit, by Roche (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Collagenase IV Collagenase

2 protocols using collagenase 4 digestion buffer

1

Isolation and Analysis of Dermal Lymphatic Endothelial Cells

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Dermal LECs were isolated from the abdominal skin by digestion in 0.4% Collagenase IV digestion buffer (MP Biomedicals, Solon, OH) and flow cytometry to sort for LECs (CD45−, CD31+, podoplanin+).(17 (link)) RNA was extracted using TRIZOL (Invitrogen, Life Technologies, Carlsbad, CA) and converted to cDNA with TaqMan reverse transcriptase kit (Roche, Branchburg, NJ). Relative gene expression between groups was analyzed by normalizing gene expression with GAPDH.(18 (link))
García Nores G.D., Cuzzone D.A., Albano N.J., Hespe G.E., Kataru R.P., Torrisi J.S., Gardenier J.C., Savetsky I.L., Aschen S.Z., Nitti M.D, & Mehrara B.J. (2016). Obesity but not high-fat diet impairs lymphatic function. International Journal of Obesity (2005), 40(10), 1582-1590.
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2

Isolation and Analysis of Dermal LECs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dermal LECs were isolated from the abdominal skin by digestion in 0.4% Collagenase IV digestion buffer (MP Biomedicals, Solon, OH, USA) and flow cytometry to sort for LECs (CD45−, CD31+, podoplanin+).17 (link) RNA was extracted using TRIZOL (Invitrogen, Life Technologies, Carlsbad, CA, USA) and converted into cDNA with TaqMan reverse transcriptase kit (Roche, Branchburg, NJ, USA). Relative gene expression between groups was analyzed by normalizing gene expression with GAPDH.18 (link)
García Nores G.D., Cuzzone D.A., Albano N.J., Hespe G.E., Kataru R.P., Torrisi J.S., Gardenier J.C., Savetsky I.L., Aschen S.Z., Nitti M.D, & Mehrara B.J. (2016). Obesity but not high-fat diet impairs lymphatic function. International Journal of Obesity (2005), 40(10), 1582-1590.
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