Disodium hydrogen phosphate

Manufactured by Tianjin Damao

Sourced in China

Disodium hydrogen phosphate is an inorganic chemical compound with the formula Na2HPO4. It is a white crystalline solid that is soluble in water and has a mildly alkaline pH. The primary function of disodium hydrogen phosphate is as a pH buffer, maintaining a stable pH in various applications.

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7 protocols using disodium hydrogen phosphate

Antioxidant Properties of Monk Fruit Extract

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Siraitia grosvenorii seed was bought from Guilin Monk Fruit Corp. Wheat flour was bought from Wudeli Flour Group Co., Ltd. (Xinxiang, China). Yeast was purchased from Angel Yeast Co., Ltd. (Yichang, China). 2, 20-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), rutin, and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich (St. Louis, MO, USA), while potassium sulfate was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Zinc sulfate, potassium ferrocyanide, absolute ethanol, petroleum ether, hydrochloric acid, sodium dihydrogen phosphate, and disodium hydrogen phosphate were acquired from Tianjin Damao Chemical Reagent Factory (Tianjin, China). All other chemicals were analytical grade.

Zhou W., Guo S., Zhang S., Lu Z., Sun Z., Ma Y., Shi J, & Zhang H. (2023). Effects of Siraitia grosvenorii seed flour on the properties and quality of steamed bread. Frontiers in Nutrition, 10, 1249639.

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Enzymatic Assay of β-D-Glucosidase

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β-D-glucosidase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., Shanghai, China; methanol (chromatographic pure), ethyl acetate (chromatographic pure), citric acid, disodium hydrogen phosphate, and anhydrous sodium carbonate were obtained from Tianjin Damao Chemical Reagent Factory, Tianjin, China; concentrator plus vacuum concentrator, ultra-low temperature refrigerator, electronic balance, ultrasonic cleaner, pipette gun were obtained from Eppendorf, Hamburg, Germany; thermostatic bath was obtained from PolyScience, Niles, IL, USA; acid meter, Trace1300 gas chromatograph, Trace ISQ mass spectrometer (GC-MS), and NanoDrop 2000 nucleic acid analyzer were obtained from Thermo, Waltham, MA, USA; high-efficiency plant total RNA extraction kit, UnionScript First-strand cDNA synthesis mix for qPCR kit, and real-time fluorescence quantitative SYBR GreenⅠMaster kit were purchased from Beijing Jinsha Biological Co., Ltd., Beijing, China; LightCycler 480 quantitative PCR instrument was obtained from Roche, Basel, Switzerland.

Jia H., Geng X., Fan L., Li X., Wang J, & Hao R. (2024). Spatial and Temporal Disparity Analyses of Glycosylated Benzaldehyde and Identification and Expression Pattern Analyses of Uridine Diphosphate Glycosyltransferase Genes in Prunus mume. Plants, 13(5), 703.

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Water Hyacinth Adsorption Protocol

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Water hyacinth was collected from a natural lake in Shantou, Guangdong. Zinc sulfate (ZnSO₄, ≥99.5%), hydrochloric acid (HCl, 36–38%), potassium hydroxide (KOH, ≥85%) and sodium thiosulfate (Na₂O₃S₂, ≥99%) were purchased from China Shanghai Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Tetracycline hydrochloride (TC, 99.9%), sodium persulfate (Na₂S₂O₈) (AR, 99%), sodium bicarbonate (NaHCO₃, ≥99.5%), humic acids (HA, ≥90%), 5,5-dimethyl-1-pyrrolidine N-oxide (DMPO, ≥97%) and 2,2,6,6-tetramethylpiperidine (TEMP, ≥97%) were purchased from Macklin Biochemical Co., Ltd (Shanghai, China). Absolute ethanol (EtOH, 99.7%) (AR), tert-butyl alcohol (TBA, ≥99%) and l-histidine (L-H, ≥99%) were purchased from Aladdin (Shanghai, China). Sodium sulfate (Na₂SO₄, ≥99%) (AR) and sodium chloride (NaCl, ≥99.5%) (AR) were purchased from Chongqing Chuandong Chemical (Group) Co., Ltd. Sodium hydroxide (NaOH, ≥96%) (AR) was purchased from Chengdu Kelong Chemical Reagent Factory. Disodium hydrogen phosphate (Na₂HPO₄·12H₂O, ≥99%) was purchased from Tianjin Damao Chemical Reagent Factory. All chemicals were analytical grade and can be used without further purification. Unless otherwise noted, deionized water was used for all solutions.

Qin Y., Wang S., Zhang B., Chen W., An M., Yang Z., Gao H, & Qin S. (2024). Zinc and sulfur functionalized biochar as a peroxydisulfate activator via deferred ultraviolet irradiation for tetracycline removal. RSC Advances, 14(8), 5648-5664.

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Purification and Characterization of CGA Compounds

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CGA, CGA-ME, and CGA-LT were purified by us using D101 macroporous resin, Sephadex LH-20 and ODS column chromatography, and preparative HPLC from the aqueous extract of FE; the compounds were identified by UV, IR, MS, and NMR technologies [14 (link)]. Their purities above 95% were determined by UPLC analysis. LC-grade acetonitrile was purchased from Sigma-Aldrich (St. Louis, MO, USA). LC-grade water was produced by a Milli-Q water purification system (Millipore, Massachusetts, MA, USA). Formic acid was bought from Meridian Medical Technologies (MREDA, New York, NY, USA). Phosphoric acid, disodium hydrogen phosphate, potassium dihydrogen phosphate, and dipotassium hydrogen phosphate were bought from DAMAO Chemical Reagent Factory (Tianjin, China).
The phosphate buffer solution at pH 2, 4, 6, and 8 was prepared according to the guidelines released by Chinese pharmacopoeia.

Yu H., Yang J., Ding J., He Y., Jiang Z., Chai X, & Wang Y. (2018). Stability Study and Identification of Degradation Products of Caffeoylgluconic Acid Derivatives from Fructus Euodiae. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(8), 1975.

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Analytical Reagent Grade Chemicals

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All reagents were of analytical reagent grade and were used without further purification. Urea was purchased from the Hedong District of the Tianjin Hongyan Reagent Factory (Tianjin, China). Sodium citrate was purchased from the Beijing Chemical Factory (Beijing, China). p-Acetaminophenol and quinine sulfate were purchased from Aladdin Co., Ltd. (Shanghai, China). Sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, H₂O₂, and chromium chloride (CrCl₃·6H₂O) were purchased from the Tianjin Damao Chemical Reagent Factory (Tianjin, China). The preparation reagents for the metal ion solutions were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shenyang, China). Anhydrous ethanol was purchased from Xinxiang Zhengxin Chemical Co., Ltd. (Henan, China).

Xu X., Li H., Sun Y., Ma T., Shi L., Mu W., Wang H, & Lu Y. (2023). Novel “on–off” fluorescence sensing for rapid and accurate determination of Cr3+ based on g-CNQDs. RSC Advances, 13(41), 28550-28559.

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Antioxidant Activities of Polyphenolic Compounds

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Quercetin, morin, festin, myricetin, rutin, and breviscapine were purchased from DATIAN Biology Co (Shanxi, China), β-arbutin from Beijing Brilliance Biochemical Co, Ltd (Beijing, China), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) from Guangzhou Qiyun Bio-technology Co (Guangzhou, China). Sodium dihydrogen phosphate, disodium hydrogen phosphate, dimethyl sulfoxide (DMSO), and absolute ethyl alcohol were purchased from DAMAO CHEMICAL REAGENT FACTORY (Tianjin, China). Span60 was purchased from Tianjin Kemiou Chemical Reagent Co, Ltd (Tianjin, China) and Cremophor RH40 from Guangzhou Jiefu Trading Co, Ltd (Guangzhou, China). Male Sprague-Dawley rats (200 ± 20 g) were used and supplied by the Guangdong Animal Experiment Center (Guangzhou, China). Rats were kept under standard temperature, humidity, and light conditions, with a standard rodent diet and water. The research was approved by Guangdong Pharmaceutical University Experimental Animal Ethics Committee (NO. SPF2017120, Guangzhou, China).

Lu B., Huang Y., Chen Z., Ye J., Xu H., Chen W, & Long X. (2019). Niosomal Nanocarriers for Enhanced Skin Delivery of Quercetin with Functions of Anti-Tyrosinase and Antioxidant. Molecules, 24(12), 2322.

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Naringinase Production from Aspergillus niger

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Aspergillus niger FFCC uv-11 was stored in our laboratory, and the naringinase fermentation broth was produced via the strain with a naringinase activity of 650.0 U mL⁻¹. Naringin (mass fraction ≥ 98%) was purchased from Baoji Fang Sheng Biological development Co., Ltd, China. Polyethyleneimine (M.W. 10 000 Da, 99%) was obtained from Aladdin Inc. Sodium hydroxide, anhydrous ethanol, citric acid, disodium hydrogen phosphate, diethylene glycol, glutaraldehyde (50% by mass), trisodium titrate and ammonia (NH₃·H₂O, 25%, w/v) were purchased from Tianjin Damao Chemical Reagent Co., Ltd. Anhydrous ferric chloride (FeCl₃) and ferrous sulfate heptahydrate (FeSO₄·7H₂O) were supplied by Sinopharm Chemical Reagent Co., Ltd.

Yu C., Li Q., Tian J., Zhan H., Zheng X., Wang S., Sun X, & Sun X. (2021). A facile preparation of immobilized naringinase on polyethyleneimine-modified Fe3O4 magnetic nanomaterials with high activity. RSC Advances, 11(24), 14568-14577.

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