A total of 1×105 cells were treated with DMEM culture without FBS for 24 hours to synchronize cell cultures. The culture medium was then substituted with DMEM culture medium which contained 10% FBS. Cells were harvested after 48 hours and fixed in 75% ethanol at −20°C for 24 hours. Afterwards, cells were rinsed in PBS three times, rehydrated and resuspended in propidium iodide (PI)-RNase A solution (Invitrogen, USA) at 37°C for 30 minutes. Cell cycle was analyzed with a FACSCalibur flow cytometer (Biosciences, San Jose, CA, USA).
Pi rnase a solution
Manufactured by Thermo Fisher Scientific
Sourced in United States
The PI)-RNase A solution is a laboratory reagent used for the digestion and degradation of RNA molecules. It contains the enzyme ribonuclease A (RNase A), which specifically cleaves single-stranded RNA.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pi rnase a solution
1
Cell Cycle Analysis of Synchronized Cultures
2
Cell Cycle Synchronization and Analysis
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Cells were incubated in DMEM medium without FBS for 24 h to synchronize cell growth, and the medium was then exchanged for DMEM with 10% FBS. After 48 h culture, the cells were fixed in 75% ethanol at −20°C for 24 h, washed with PBS three times, resuspended in propidium iodide (PI)-RNase A solution (Invitrogen, USA), and incubated at 37°C for 30 min. Cell cycle distribution was analyzed using a FACScan flow cytometer (BD Biosciences, San Jose, CA, USA).
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