Cells were plated in 96-well plates (2 K cells/well) in 8 replicate. The cells were harvested at day 1, day 3, and day 5 in PBS buffer. The cells then underwent three rounds of standard freeze and thaw, followed ny staining with Hoechst 33342 (10 µM in PBS buffer). The fluorescence intensity of Hoechst 33342 was determined using a BioTek (Winooski, VT, USA) Mx microplate reader with an excitation of 361 nm and an emission of 497 nm. Average relative fluorescence intensity (day 1 control conditions are set as 1) of each experimental conditions was presented as graphs with SD indicated.
Mx microplate reader
Manufactured by Agilent Technologies
Sourced in United States
The Mx microplate reader is a versatile laboratory instrument designed for a wide range of applications. It is capable of performing absorbance, fluorescence, and luminescence measurements on 96- and 384-well microplates. The Mx microplate reader provides accurate and reproducible results for various assays, including enzyme-linked immunosorbent assays (ELISA), cell-based assays, and DNA/RNA quantification.
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Lab products found in correlation
2 protocols using mx microplate reader
1
Quantifying Cell Viability by Hoechst Staining
2
Cell Viability Assay with Hoechst 33342
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Cells were plated in 96-well plates (2 K cells/well) in quadruplicate and treated with vehicle or the indicated drugs. The cells were harvested at day 1, day 4, and day 6 in PBS buffer. The cells then underwent three rounds of standard freeze and thaw followed staining with Hoechst 33342 (10 µM in PBS buffer). The fluorescence intensity of Hoechst 33342 was determined using a BioTek Mx microplate reader with an excitation of 361 nm and an emission of 497 nm. Average relative fluorescence intensity (day 1 vehicle-treated conditions are set as 1) of each treatment condition was presented as graphs with SD indicated.
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