Chloramphenicol

Manufactured by MedChemExpress

Sourced in China, United States

Chloramphenicol is a synthetic antibacterial agent. It is a white crystalline powder that is soluble in alcohol, chloroform, and water. Chloramphenicol functions as an inhibitor of protein synthesis in bacteria by preventing the translocation step in protein synthesis.

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Lab products found in correlation

3 protocols using chloramphenicol

Enterococcus faecalis Isolates from China

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A total of 302 non-duplicate E. faecalis isolates were collected from different inpatients at Shenzhen Nanshan People’s Hospital (Grade A, level III Hospital, 1500 beds), Shenzhen University, China, between January 1, 2011, and December 31, 2016. These E. faecalis isolates were obtained from urine (135 isolates), blood (37 isolates), pus or secretions (86 isolates), bile (25 isolates), and other clinical sources (19 isolates). Based on a previous study, the dominant multilocus sequence types (MLSTs) of these isolates were ST16 and ST179 (Zheng et al., 2017 (link)). The isolates were identified by the Phoenix 100 automated microbiology system (BD, Franklin Lakes, NJ, United States), following which two subcultured generations of all the 302 isolates were re-identified with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (IVD MALDI Biotyper, Bruker, Bremen, Germany). E. faecalis strains ATCC29212 and OG1RF (ATCC47077) were used as reference strains.
Chloramphenicol (catalog no. HY-B0239), linezolid (catalog no. HY-10394), and radezolid (catalog no. HY-14800) were purchased from MedChemExpress (MCE, Shanghai, China).

Zheng J., Chen Z., Lin Z., Sun X., Bai B., Xu G., Chen J., Yu Z, & Qu D. (2020). Radezolid Is More Effective Than Linezolid Against Planktonic Cells and Inhibits Enterococcus faecalis Biofilm Formation. Frontiers in Microbiology, 11, 196.

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Antimicrobial Susceptibility Testing Protocol

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AF and antibiotics [fosfomycin (FOF); ciprofloxacin (CIP); tetracycline (TET); linezolid (LZD); chloramphenicol (CHL); levofloxacin (LVX); teicoplanin (TEC); clindamycin hydrochloride (CLI) hydro; gentamicin (GEN); vancomycin (VAN)] were purchased from the MedChemExpress company (Monmouth Junction, NJ, United States). E. faecalis ATCC 29212, S. aureus ATCC25923 and ATCC29213 were kindly provided by Juncai Luo (Tiandiren Biotech, Changsha, China). S. epidermidis RP62A and ATCC 12228 were given by Di Qu (Shanghai Medical College of Fudan University), S. aureus ATCC43300 (MRSA), Newman, and RJ-2 were given by Min Li (Renji Hospital, Shanghai Jiao Tong University School of Medicine). Other clinical strains were isolated from the wound secretion or sputum of inpatients at the Third Xiangya Hospital of Central South University. Staphylococcus spp. were grown in tryptic soy broth (TSB) broth medium (Solarbio, Shanghai, China), and E. faecalis was grown in brain heart infusion (BHI) broth medium (Solarbio, Shanghai, China) at 37°C.

She P., Zhou L., Li S., Liu Y., Xu L., Chen L., Luo Z, & Wu Y. (2019). Synergistic Microbicidal Effect of Auranofin and Antibiotics Against Planktonic and Biofilm-Encased S. aureus and E. faecalis. Frontiers in Microbiology, 10, 2453.

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Enterobacter cloacae Strain Cultivation

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All strains, plasmids, and primers used in this study are listed in Table 1 and 2. We used the Enterobacter cloacae type strain ATCC13047 and its derivatives for all experiments. All strains were initially grown from freezer stocks on solid Luria-Bertani (LB) agar at 37°C. Isolated colonies were used to inoculate LB at 37°C. Where applicable, kanamycin was used at 50 μg/mL, chloramphenicol at 100 μg/mL, and cecropin B (MedChemExpress, Monmouth Junction, NJ) at 20 μg/mL unless otherwise noted. For complementation experiments, 0.2% arabinose or 200 μM Isopropyl-ß-D-thiogalactopyranoside (IPTG) was used to induce plasmid-borne genes.

Murtha A.N., Kazi M., Kim E., Rosch K.M., Torres F, & Dörr T. (2024). Multiple resistance factors collectively promote inoculum-dependent dynamic survival during antimicrobial peptide exposure in Enterobacter cloacae. bioRxiv.

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