Phosphatase inhibitor set

Manufactured by Merck Group

The Phosphatase inhibitor set is a collection of chemical compounds designed to inhibit the activity of phosphatase enzymes. Phosphatases play a crucial role in various biological processes, and their inhibition can be valuable in research and experimental applications.

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Lab products found in correlation

Complete protease inhibitor set, by Merck Group (2 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions)

Close spelling variants of this product

Phosphatase Inhibitor Cocktail Sets 1 and 2 Phosphatase Inhibitor Set II Phosphatase Inhibitor Cocktail Set II Phosphatase inhibitor cocktail set IV Phosphatase inhibitor cocktail set V Phosphatase Inhibitor Cocktail Set I Phosphatase inhibitor cocktail set Protein phosphatase inhibitor set Phosphatase inhibitor cocktail set I and II Phosphatase Inhibitor Cocktail Set II 1×

3 protocols using phosphatase inhibitor set

Western Blotting of Cellular and Tissue Samples

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Western blotting was performed as previously described (Wang et al., 2014 (link)). Briefly, cell pellet samples were collected and re-suspended in lysis buffer (100 mM Tris-HCl, pH 7.4, 100 mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA, Roche complete protease inhibitor set, and Sigma phosphatase inhibitor set), incubated on ice for 30 min, and centrifuged at 20,000 × g for 30 min. The supernatants were collected for western blotting. Testes tissue samples were ground and re-suspended in lysis buffer, homogenized for 30 s with a Paddle Blender (Prima, PB100), incubated on ice for 30 min, and centrifuged at 20,000 × g for 30 min. The supernatants were collected for western blotting.

Li D., Meng L., Xu T., Su Y., Liu X., Zhang Z, & Wang X. (2017). RIPK1-RIPK3-MLKL-dependent necrosis promotes the aging of mouse male reproductive system. eLife, 6, e27692.

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Cell Lysis and Protein Extraction

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Cell pellets were collected and resuspended in lysis buffer (20 mM Tris-HCl, pH 7.4,150 mM NaCl, 10% glycerol, 1% Triton X-100, 1 mM Na₃VO₄, 25 mM β-glycerol-phosphate, 0.1 mM PMSF, with a complete Roche protease inhibitor set and a Sigma phosphatase inhibitor set). The resuspended cell pellet or tissue was vortexed for 10 s, incubated on ice for 20 min, and centrifuged at 20,000 × g for 20 min. The supernatants were collected for western blot analysis or immunoprecipitation. Images have been cropped for presentation. Full-size images are presented in Supplementary Fig. 15.

Dong T., Li C., Wang X., Dian L., Zhang X., Li L., Chen S., Cao R., Li L., Huang N., He S, & Lei X. (2015). Ainsliadimer A selectively inhibits IKKα/β by covalently binding a conserved cysteine. Nature Communications, 6, 6522.

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Immunoprecipitation and Western Blot Analysis

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Cell pellets were collected and resuspended in lysis buffer (20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 10% glycerol, 1% Triton X-100, 1 mM Na₃VO₄, 25 mM β-glycerolphosphate, 0.1 mM PMSF, Roche complete protease inhibitor set, and Sigma–Aldrich phosphatase inhibitor set). The resuspended cell pellet was vortexed for 20 s and then incubated on ice for 30 min and centrifuged at 20 000×g for 30 min. The supernatants were collected for western blot analysis.
For immunoprecipitation, anti-Snail-1 antibody or anti-13–3-3 zeta and their isotype control antibodies were coupled to protein A agarose beads (Pierce) in PBS containing 5 mg/ml bovine serum albumin (Sigma–Aldrich) for 6 h at 4°C on a rotating platform. The cell lysates were then incubated with the beads coupled with antibodies overnight at 4°C. The next day, beads were washed with lysis buffer containing an additional 300 mM NaCl, and the immune complexes were eluted off the beads using loading buffer with boiling for 5 min and then subjected to western blot analysis.

Zhang X., Li L.X., Yu C., Nath K.A., Zhuang S, & Li X. (2022). Targeting lysine-specific demethylase 1A inhibits renal epithelial–mesenchymal transition and attenuates renal fibrosis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36(1), e22122.

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