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Alt activity assay

Manufactured by Merck Group
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The ALT Activity Assay is a laboratory test used to measure the activity of the enzyme alanine aminotransferase (ALT) in a biological sample. ALT is an enzyme found primarily in the liver and is used as a biomarker to assess liver health. The assay provides quantitative information about the level of ALT activity, which can be helpful in the diagnosis and monitoring of liver-related conditions.

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Lab products found in correlation

Ast activity assay, by Merck Group (3 mentions) Vitros dt60 2, by Ortho Clinical Diagnostics (2 mentions) Mouse glucagon elisa kit, by Crystal Chem (1 mentions) Mouse glucose assay kit, by Crystal Chem (1 mentions) Mouse ultrasensitive insulin elisa kit, by ALPCO (1 mentions) Lentiviral vectors expressing, by GenePharma (1 mentions) Ast activity assay kit, by Merck Group (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) Serum separator tube, by BD (1 mentions) Creatinine assay, by Merck Group (1 mentions) Total protein kit, by Merck Group (1 mentions) Rat insulin elisa kit, by Thermo Fisher Scientific (1 mentions) Glucose assay kit, by Merck Group (1 mentions) Ctg reagent, by Promega (1 mentions) Cellplayer reagent, by Sartorius (1 mentions) Incucyte instrument, by Sartorius (1 mentions) T48402, by Merck Group (1 mentions)

Close spelling variants of this product

AST and ALT activity assays Alanine Aminotransferase (ALT) Activity Assay Kit ALT and AST activity assay kits AST and ALT Activity Assay Kit ALT activity assay kit

16 protocols using alt activity assay

1

Measuring ALT Activity in Mouse Serum

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ALT enzymatic activity was measured by an ALT activity assay (Sigma-Aldrich) in 10 µl of mouse serum according to the supplier’s instructions.
Paillet J., Plantureux C., Lévesque S., Le Naour J., Stoll G., Sauvat A., Caudana P., Tosello Boari J., Bloy N., Lachkar S., Martins I., Opolon P., Checcoli A., Delaune A., Robil N., de la Grange P., Hamroune J., Letourneur F., Autret G., Leung P.S., Gershwin M.E., Zhu J.S., Kurth M.J., Lekbaby B., Augustin J., Kim Y., Gujar S., Coulouarn C., Fouassier L., Zitvogel L., Piaggio E., Housset C., Soussan P., Maiuri M.C., Kroemer G, & Pol J.G. (2021). Autoimmunity affecting the biliary tract fuels the immunosurveillance of cholangiocarcinoma. The Journal of Experimental Medicine, 218(10), e20200853.
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2

Comprehensive Metabolic Profiling of Mice

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Serum insulin and glucagon levels were determined by the Mouse Ultrasensitive Insulin ELISA kit (ALPCO) and Mouse Glucagon ELISA Kit (Crystal Chem), respectively. In addition, the Serum Triglyceride Assay Kit (Abcam) and the ALT Activity Assay (Sigma) were used. Urine glucose levels were assessed using a Mouse Glucose Assay Kit (Crystal Chem).
Thielen L.A., Chen J., Jing G., Moukha-Chafiq O., Xu G., Jo S., Grayson T.B., Lu B., Li P., Augelli-Szafran C.E., Suto M.J., Kanke M., Sethupathy P., Kim J.K, & Shalev A. (2020). Identification of an anti-diabetic, orally available, small molecule that regulates TXNIP expression and glucagon action. Cell metabolism, 32(3), 353-365.e8.
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3

Serum Collection and ALT Assay

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Serum was collected from mice at euthanization, based on disease-burden. Whole blood was collected, allowed to sit at room temperature for 15 minutes, then centrifuged for 20 minutes at 4°C. The resulting serum was collected and stored at −80°C until use. ALT was measured using a commercially available ALT Activity Assay (Sigma-Aldrich), following the manufacturer's protocol.
Sullivan P.M., Kumar R., Li W., Hoglund V., Wang L., Zhang Y., Shi M., Beak D., Cheuk A., Jensen M.C., Khan J., Dimitrov D.S, & Orentas R.J. (2022). FGFR4-Targeted Chimeric Antigen Receptors Combined with Anti-Myeloid Polypharmacy Effectively Treat Orthotopic Rhabdomyosarcoma. Molecular Cancer Therapeutics, 21(10), 1608-1621.
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4

Serum ALT Measurement in Mice

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Alanine aminotransferase (ALT) was measured in the serum of mice using the Vitros DT60 II chemistry system (Ortho-Clinical Diagnostics, Inc.) (Zheng et al., 2016 (link)) or ALT Activity Assay (Sigma) per manufacturer’s instructions. Method of ALT measurement was consistent within experiments.
Cruz P.C., Wanek A.G., Kumar P., An X., Elsegeiny W., Horne W., Fitch A., Burr A.H., Gopalakrishna K.P., Chen K., Methé B.A., Canna S.W., Hand T.W, & Kolls J.K. (2019). Intestinal IL-17R Signaling Constrains IL-18-Driven Liver Inflammation by the Regulation of Microbiome-Derived Products. Cell reports, 29(8), 2270-2283.e7.
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5

ALT Activity Assay Protocol

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ALT tests were performed using ALT activity assay (Sigma) according to the manufacturer's protocol.
Su T., Bondar T., Zhou X., Zhang C., He H, & Medzhitov R. (2015). Two-signal requirement for growth-promoting function of Yap in hepatocytes. eLife, 4, e02948.
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6

Alanine Aminotransferase Activity Assay

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Alanine Aminotransferase Activity (ALT) was assessed on the plasma of STD and MCD diet fed mice (at 4 and 8 weeks) with the ALT activity assay (Sigma-Aldrich), according to the manufacturer protocol. Results are express in International Unit (IU)/mL.
Nachit M., Montemagno C., Clerc R., Ahmadi M., Briand F., Bacot S., Devoogdt N., Serdjebi C., Ghezzi C., Sulpice T., Broisat A., Leclercq I.A, & Perret P. (2023). Molecular imaging of liver inflammation using an anti-VCAM-1 nanobody. Nature Communications, 14, 1062.
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7

Murine Serum Collection and ALT Assay

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Serum was collected from mice at euthanization, based on disease-burden. Whole blood was collected, allowed to sit at room temperature for 15 minutes, then centrifuged for 20 minutes at 4C. The resulting serum was collected and stored at −80C until use. ALT was measured using a commercially available ALT Activity Assay (Sigma Aldrich), following the manufacturer’s protocol.
Sullivan P.M., Kumar R., Li W., Hoglund V., Wang L., Zhang Y., Shi M., Beak D., Cheuk A., Jensen M.C., Khan J., Dimitrov D.S, & Orentas R.J. (2022). FGFR4-Targeted Chimeric Antigen Receptors Combined with Anti-Myeloid Polypharmacy Effectively Treat Orthotopic Rhabdomyosarcoma. Molecular Cancer Therapeutics, 21(10), 1608-1621.
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8

Adenovirus-Induced Liver Injury in Mice

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Groups of six C57BL/6 mice were injected i.v. with 1011 vp of Ad5, Ad6, or Ad657 by tail vein and blood was collected 3 days later for ALT measurement using ALT Activity Assay (Sigma-Aldrich, St. Louis, MO, USA).
Nguyen T.V., Crosby C.M., Heller G.J., Mendel Z.I., Barry M.E, & Barry M.A. (2018). Oncolytic adenovirus Ad657 for systemic virotherapy against prostate cancer. Oncolytic Virotherapy, 7, 43-51.
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9

Serum ALT Measurement in Mice

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Alanine aminotransferase (ALT) was measured in the serum of mice using the Vitros DT60 II chemistry system (Ortho-Clinical Diagnostics, Inc.) (Zheng et al., 2016 (link)) or ALT Activity Assay (Sigma) per manufacturer’s instructions. Method of ALT measurement was consistent within experiments.
Cruz P.C., Wanek A.G., Kumar P., An X., Elsegeiny W., Horne W., Fitch A., Burr A.H., Gopalakrishna K.P., Chen K., Methé B.A., Canna S.W., Hand T.W, & Kolls J.K. (2019). Intestinal IL-17R Signaling Constrains IL-18-Driven Liver Inflammation by the Regulation of Microbiome-Derived Products. Cell reports, 29(8), 2270-2283.e7.
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10

ALT Activity Assay for Liver Function

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Liver enzyme function was examined using the ALT Activity Assay (MAK052, Sigma-Aldrich). As a positive control, we used LPS to induce high levels of secreted ALT levels in the plasma as previously shown(Baveja et al., 2002 (link)).
Farfara D., Feierman E., Richards A., Revenko A.S., MacLeod R.A., Norris E.H, & Strickland S. (2019). Knockdown of circulating C1 inhibitor induces neurovascular impairment, glial cell activation, neuroinflammation, and behavioral deficits. Glia, 67(7), 1359-1373.
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