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Hank s balanced salt solution

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Hank's Balanced Salt Solution is a buffered saline solution commonly used as a base medium for cell culture applications. It provides a balanced salt composition that helps maintain the physiological environment for cells in culture. The solution contains inorganic salts, glucose, and phenol red as a pH indicator.

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Lab products found in correlation

Trypsin edta, by Thermo Fisher Scientific (3 mentions) Igrov1, by Corning (2 mentions) A2780, by Corning (2 mentions) Mcdb 105, by Merck Group (2 mentions) Skov3, by Merck Group (2 mentions) Skov3, by American Type Culture Collection (2 mentions) A2780, by American Type Culture Collection (2 mentions) Igrov1, by American Type Culture Collection (2 mentions) Myelocult m5300, by STEMCELL (1 mentions) Hydrocortisone, by STEMCELL (1 mentions)

4 protocols using hank s balanced salt solution

1

Isolation and Culture of Ovarian Cancer Cells

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The human OC cell lines SKOV3, IGROV1 and A2780 were from the American
Type Culture Collection (Rockville, MD, USA). De-identified OC ascites samples
were obtained through an IRB approved protocol of the Indiana University Simon
Cancer Center Tissue Bank. Ascites tumor cells were collected by centrifugation
at 200 × g for 3 min. Erythrocytes were lysed by re-suspending the cell
pellet in a 1:4 mixture of cold Hank's balanced salt solution modified (StemCell
Technologies) supplemented with 2% FBS and red blood cell lysis buffer (0.8%
ammonium chloride, 0.1 mM EDTA, pH 7.4) for 5 min. After centrifugation at 350
× g for 5 min, 25,000 ascites derived tumor cells were cultured as
monolayers or spheroids. SKOV3 and primary OC cells were cultured in media
containing 1:1 MCDB 105 (Sigma) and M199 (Cellgro, Herndon, VA, USA)
supplemented with 10% FBS and antibiotics, while IGROV1 and A2780 cells were
grown in RPMI 1640 at 37°, under a humidified atmosphere containing 5%
CO2.
Condello S., Morgan C.A., Nagdas S., Cao L., Turek J., Hurley T.D, & Matei D. (2014). Beta-Catenin Regulated ALDH1A1 is a Target in Ovarian Cancer Spheroids. Oncogene, 34(18), 2297-2308.
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2

Bile Acid Effects on Bone Marrow Cells

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Irradiated AFT024 cells were seeded to confluency in 24-well plates in Dulbecco modified Eagle medium + 10% FBS + 0.05 mM 2-mercaptoethanol in a 33°C, 5% CO2 incubator. Complete media was made adding 0.22 μm filter–sterilized minimum essential medium α with nucleosides (StemCell Technologies) containing 1mM hydrocortisone (StemCell Technologies) to Myelocult M5300 (StemCell Technologies) for a final concentration of 1 μM hydrocortisone. AFT024 media was removed from the wells, and 200 000 whole bone marrow cells in 1 mL of complete media were added to each well. Cells were cultured for 1 week either in control media or in the presence of DCA (35 μM) or LCA (18 μM). After 1 week, media were removed and placed into a collection tube, adherent cells were washed with Hanks balanced salt solution (StemCell Technologies). Hanks balanced salt solution was removed and placed into the collection tube, and cells were treated with Trypsin-EDTA (0.05%; Thermo Fisher Scientific). Trypsinization was halted by washing cells with heat-inactivated FBS, followed by IMDM + 2% FBS. This was placed back into the collection tube and centrifuged to form pellet cells. The media were decanted via aspiration. Cells were resuspended in FACS buffer (PBS + 1% FBS) and stained for spectral flow cytometry.
Thompson B., Lu S., Revilla J., Uddin M.J., Oakland D.N., Brovero S., Keles S., Bresnick E.H., Petri WA J.r, & Burgess S.L. (2023). Secondary bile acids function through the vitamin D receptor in myeloid progenitors to promote myelopoiesis. Blood Advances, 7(17), 4970-4982.
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3

Mechanical Dissociation of Keratinocyte Monolayers

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Dispase-based mechanical dissociation assays were carried out as described (104 (link)). Keratinocytes were plated at a density of 1 × 106 cells per well of 6-well cell culture dishes. Upon reaching confluence, the calcium concentration of the medium was adjusted to 1.3 mM. Vehicle control (DMSO) or chemical inhibitors were added at the following concentrations: thapsigargin (1 μM), dabrafenib (1 μM), trametinib (1 μM), U0126 (10 μM), PD184352 (10 μM), or PD98059 (20 μM). After 24 to 72 hours, monolayers were washed with PBS and then incubated with 500 μL dispase (5 U/mL) in Hank’s balanced salt solution (StemCell Technologies, catalog 07913) for 30 minutes at 37°C. Next, 4.5 mL PBS was added to the wells, and all liquid plus released monolayers were transferred into 15 mL conical tubes, which were placed together in a rack and inverted 5–10 times to induce mechanical stress. Monolayer fragments were transferred back into 6-well cell culture plates and imaged with a 12-megapixel digital camera. Fragments were counted in Fiji.
Zaver S.A., Sarkar M.K., Egolf S., Zou J., Tiwaa A., Capell B.C., Gudjonsson J.E, & Simpson C.L. (2023). Targeting SERCA2 in organotypic epidermis reveals MEK inhibition as a therapeutic strategy for Darier disease. JCI Insight, 8(18), e170739.
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4

Isolation and Culture of Ovarian Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human OC cell lines SKOV3, IGROV1 and A2780 were from the American
Type Culture Collection (Rockville, MD, USA). De-identified OC ascites samples
were obtained through an IRB approved protocol of the Indiana University Simon
Cancer Center Tissue Bank. Ascites tumor cells were collected by centrifugation
at 200 × g for 3 min. Erythrocytes were lysed by re-suspending the cell
pellet in a 1:4 mixture of cold Hank's balanced salt solution modified (StemCell
Technologies) supplemented with 2% FBS and red blood cell lysis buffer (0.8%
ammonium chloride, 0.1 mM EDTA, pH 7.4) for 5 min. After centrifugation at 350
× g for 5 min, 25,000 ascites derived tumor cells were cultured as
monolayers or spheroids. SKOV3 and primary OC cells were cultured in media
containing 1:1 MCDB 105 (Sigma) and M199 (Cellgro, Herndon, VA, USA)
supplemented with 10% FBS and antibiotics, while IGROV1 and A2780 cells were
grown in RPMI 1640 at 37°, under a humidified atmosphere containing 5%
CO2.
Condello S., Morgan C.A., Nagdas S., Cao L., Turek J., Hurley T.D, & Matei D. (2014). Beta-Catenin Regulated ALDH1A1 is a Target in Ovarian Cancer Spheroids. Oncogene, 34(18), 2297-2308.
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