RNA was prepared from A2780, A2780pacR and A2780olapR cells and integrity was assessed as described above. Each RNA sample was converted to biotinylated amplified cRNA using an Illumina TotalPrep RNA Amplification Kit (Life Technologies) according to the manufacturer's guidelines, and cRNA quality and concentration were confirmed on an Agilent Bioanalyzer 2100 (Agilent Technologies, Wokingham, UK). cRNA samples were hybridised in triplicate on Illumina Human HT-12 BeadChip Arrays (Illumina, Little Chesterford, UK) using standard protocols optimised by the Genetics Core, Wellcome Trust Clinical Research Facility, University of Edinburgh.
Human ht 12 beadchip arrays
Manufactured by Illumina
Sourced in United Kingdom
The Human HT-12 BeadChip Arrays are a high-throughput gene expression profiling platform developed by Illumina. The arrays contain more than 47,000 probes, enabling the comprehensive analysis of the human transcriptome. The core function of the Human HT-12 BeadChip Arrays is to provide a robust and efficient method for parallel measurement of gene expression levels across a large number of samples.
Automatically generated - may contain errors
3 protocols using human ht 12 beadchip arrays
1
Transcriptome Analysis of Chemoresistant Ovarian Cancer Cells
2
Quantifying DICER1 and GAPDH Expression in PTSD and Depression
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In a subset of 40 subjects (20 cases of PTSD&Dep and 20 controls), whose RNA was run on the Illumina HumanHT12 Beadchip arrays, total RNA from whole blood was reverse transcribed into cDNA using the RT2 First Strand Kit (Qiagen). DICER1 and GAPDH as housekeeping control were detected and quantified in duplicates using the TaqMan assays Hs00229023_m1 (DICER1) and Hs02758991_g1 (GAPDH) with TaqMan Gene Expression Master Mix on a ViiA7 real-time PCR system in 384-well format according to the instruction of the manufacturer (Applied Biosciences). Gene expression differences and fold changes were calculated using the 2−ΔΔCt method62 (link).
3
Illumina Human HT-12 BeadChip Protocol
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RNA was prepared from A2780 and A2780/GSTP1 cells and integrity assessed as described above. Each RNA sample was converted to biotinylated amplified cRNA using an Illumina TotalPrep RNA Amplification kit (Life Technologies) according to the manufacturer's guidelines, and cRNA quality and concentration confirmed on the Agilent Bioanalyzer 2100, as described above. cRNA samples were hybridised in triplicate on Illumina Human HT-12 BeadChip Arrays (Illumina, Little Chesterford, UK) using standard protocols optimised by the Genetics Core, Wellcome Trust Clinical Research Facility, University of Edinburgh.
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