Embryo medium was made by dissolving 0.60 g of Instant Ocean® Sea Salt (Blacksburg, VA, United States) and 0.038 g of sodium bicarbonate (Sigma, Diegem, Belgium) in 2 L reverse osmosis (RO) water (pH 7.4 ± 0.3) (Barnstead™ Pacific™ RO Water Purification System, Thermo Scientific™, Waltham, MA, United States ). The MS-222 solution (1 g/L) was made by dissolving methyl ethane sulfonate (i.e. MS-222) (Sigma) in embryo medium, and the pH was adjusted to 7.4 ± 0.3 with 1M NaOH. For the first experiment, DMSO (Sigma) was added to embryo medium to obtain the different DMSO concentrations (0.01%, 0.1%, 0.5%, 1% and 2%). For the second experiment, the following solutions were prepared: embryo medium (medium control), a 1% DMSO (Sigma) solution in embryo medium (solvent control), a 100 µM ascorbic acid (AA) (Sigma) solution in embryo medium, a 100 µM AA (Sigma) solution in embryo medium containing 1% DMSO and a 1,000 µM hydrochlorothiazide (HCT) (Sigma) solution in embryo medium containing 1% DMSO.
Hydrochlorothiazide hct
Manufactured by Merck Group
Sourced in United States
Hydrochlorothiazide (HCT) is a diuretic medication used to treat high blood pressure and fluid retention. It works by increasing the amount of salt and water the kidneys remove from the body.
Automatically generated - may contain errors
Lab products found in correlation
Sodium bicarbonate, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Ms 222, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Instant ocean sea salt, by Merck Group (1 mentions)
Furosemide, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Barnstead nanopure diamond, by Thermo Fisher Scientific (1 mentions)
2 protocols using hydrochlorothiazide hct
1
Embryo Medium Composition and Treatments
2
Quantification of Furosemide and Hydrochlorothiazide
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Standard solutions of furosemide and hydrochlorothiazide (HCT; purities ≥98% and ≥99%, respectively) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Methanol (purity LC–MS) was obtained from JT Baker® (Avantor Performance Materials, Inc., Center Valley, PA, USA). Formic acid (purity approximately 98%) was obtained from Sigma-Aldrich. The water used, HPLC-grade, was obtained from distilled water for purification, via the purification system Barnstead Nanopure Diamond (Thermo Fisher Scientific, Palo Alto, CA, USA). Stock solutions of furosemide and HCT (used as internal standard) were obtained by dissolving 15.0 mg of standard solids, accurately weighed, in 100 mL of methanol, obtaining solutions of 150 mg L−1 (150 ppm) concentration, which were stored at −20 °C. Working solutions used for the analysis in LC–MS/MS or for the creation of urine samples fortified were obtained starting from stock solutions by dilution with water or methanol, using a dilution factor less than 10 in each step of dilution.
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