Human aortic (HAECs) and umbilical (HUVEs) endothelial cells (Lonza®) were cultured in EGM-2 (Lonza®) containing 100 U/mL penicillin, 100 mg/mL streptomycin, 10 % of FBS and incubated at 37 °C with 5 % of CO2 in a water saturated atmosphere. Cells were used between passages 4–8. PTP1B expression was repressed via viral infection of 90 % confluent cells with either a scrambled or PTP1B siRNA (Silencer Select siRNA PTPN1, Invitrogen®, catalogue# 1105) in the presence of Lipofectamine RNAiMAX (Invitrogen®). After 3 days, cells were treated or not for 24 h with tunicamycin (5 μg/mL) and used for MTT or western blotting assay.
Silencer select sirna ptpn1
Manufactured by Thermo Fisher Scientific
Silencer Select siRNA PTPN1 is a laboratory reagent designed to target the PTPN1 gene. It is used in RNA interference (RNAi) experiments to reduce the expression of the PTPN1 gene in cultured cells.
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Lab products found in correlation
3 protocols using silencer select sirna ptpn1
1
Modulating PTP1B expression in endothelial cells
2
Modulating PTP1B expression in endothelial cells
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Human aortic (HAECs) and umbilical (HUVEs) endothelial cells (Lonza®) were cultured in EGM-2 (Lonza®) containing 100 U/mL penicillin, 100 mg/mL streptomycin, 10 % of FBS and incubated at 37 °C with 5 % of CO2 in a water saturated atmosphere. Cells were used between passages 4–8. PTP1B expression was repressed via viral infection of 90 % confluent cells with either a scrambled or PTP1B siRNA (Silencer Select siRNA PTPN1, Invitrogen®, catalogue# 1105) in the presence of Lipofectamine RNAiMAX (Invitrogen®). After 3 days, cells were treated or not for 24 h with tunicamycin (5 μg/mL) and used for MTT or western blotting assay.
3
PTP1B Silencing in Aortic Endothelial Cells
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Human aortic endothelial cells (HAECs) were purchased from Lonza and grown (passage 3–9) in EBM2 medium containing 100 U/ml penicillin, 100 mg/ml streptomycin, and 10% FBS. PTP1B expression was repressed via viral infection of 90% confluent cells with either a control or PTP1B siRNA (Silencer Select siRNA PTPN1, Invitrogen, catalogue# 1105) in the presence of Lipofectamine RNAiMAX (Invitrogen). Cells were exposed to either a low (5 mM) or high glucose (25 mM) concentration for 5 days to mimic diabetic conditions. At the end of the experiments cells were harvested for Western-blot.
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