Purified water was obtained by using a Milli-Q Integral 3 system (Millipore SAS, Molsheim, France). Zomepirac sodium salt (used as internal standard, IS), L-pyroglutamic acid, hypoxanthine, L-histidine monohydrochloride monohydrate, oleic acid, L(+) lactic acid, LS-MS grade acetonitrile, methanol, formic acid, and LC grade ethanol were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). The API-TOF reference mass solution kit (G1969-850001) and tuning solutions, ESI-L low-concentration tuning mix (G1969-85000), and ESI-TOF Biopolymer Analysis reference masses (G1969-850003) were purchased from Agilent Technologies (Santa Clara, CA, USA).
Api tof reference mass solution kit
Manufactured by Agilent Technologies
Sourced in United States
The API-TOF reference mass solution kit is a set of premixed solutions designed for use with Agilent's time-of-flight (TOF) mass spectrometers. It provides a set of known reference masses to assist with accurate mass calibration and verification of instrument performance.
Automatically generated - may contain errors
Lab products found in correlation
Esi tof biopolymer analysis reference masses, by Agilent Technologies (6 mentions)
Formic acid, by Merck Group (5 mentions)
Milli q integral 3 system, by Merck Group (4 mentions)
Zomepirac sodium salt, by Merck Group (4 mentions)
Esi l low concentration tuning mix g1969 85000, by Agilent Technologies (4 mentions)
Ls ms grade acetonitrile, by Merck Group (3 mentions)
Lc grade ethanol, by Merck Group (3 mentions)
Methanol, by Merck Group (3 mentions)
Lc ms grade methanol, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Ethanol, by Merck Group (2 mentions)
Esi l low concentration tuning mix, by Agilent Technologies (2 mentions)
Pure p a ammonium solution, by Avantor (2 mentions)
Hypoxanthine, by Merck Group (1 mentions)
L lactic acid, by Merck Group (1 mentions)
L pyroglutamic acid, by Merck Group (1 mentions)
L histidine monohydrochloride monohydrate, by Merck Group (1 mentions)
Oleic acid, by Merck Group (1 mentions)
Silylation grade pyridine, by Avantor (1 mentions)
Milli q plus185 system, by Merck Group (1 mentions)
9 protocols using api tof reference mass solution kit
1
Characterization of Biological Compounds
2
Mass Spectrometry Reagent Standards
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Purified water was obtained using the Milli-Qplus185 system (Millipore, Billerica, MA, USA). LC–MS grade methanol (MeOH), acetonitrile (ACN), formic acid and LC grade ethanol (EtOH), standard mix for GC–MS, containing grain fatty acid methyl ester mixture (C8-C28), 2-propanol and analytical grade heptane were purchased from Sigma-Aldrich ChemieGmbH (Steinheim, Germany). C18:0methyl ester and N,O-bis(trimethylsilyl) trifluoroacetamide with 1% trimethylchlorosilane were obtained from Pierce Chemical Co (Rockford, IL, USA). Silylation grade pyridine was purchased from VWR International BHDProlabo (Madrid, Spain). The API-TOF reference mass solution kit (G1969-850001) and tuning solutions, ESI-L low concentration tuning mix (G1969-85000) and ESI-TOF Biopolymer Analysis referencemasses (G1969-850003) were purchased from Agilent Technologies (Santa Clara, CA, USA).
3
Preparation of LC-MS Grade Solvents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Purified water was obtained using the Milli-Q Integral 3 system (Millipore SAS, Molsheim, France). LC-MS grade methanol, acetonitrile, formic acid and LC grade acetone, and ethanol were purchased from Sigma-Aldrich Chemie GmbH, Steinheim, Germany. The API-TOF reference mass solution kit (G1969-850001) and tuning solutions, ESI-L low concentration tuning mix (G1969-85000), and ESI-TOF Biopolymer Analysis reference masses (G1969-850003) were purchased from Agilent Technologies, Santa Clara, California, USA.
4
Quantitative Lipidomic Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blank samples included mixtures of methanol/ethanol (1:1) that were prepared in the same way as biological samples. Zomepirac sodium salt (used as the internal standard (IS), arachidonic acid, docosahexaenoic acid, lysophosphatidylethanolamine (LPE) 18:0, phosphatidylethanolamine (PE) 16:0/22:6, LS-MS-grade acetonitrile, methanol, LC-grade formic acid, and ethanol were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). The API-TOF reference mass solution kit (G1969-850001) and tuning solutions, ESI-L low-concentration tuning mix (G1969-85000), and ESI-TOF Biopolymer Analysis reference masses (G1969-850003) were purchased from Agilent Technologies (Santa Clara, CA, United States). Purified water was obtained using the Milli-Q Integral three system (Millipore SAS, Molsheim, France) in order to prepare solution A.
5
Optimized UHPLC-HRMS Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The conditions for performing chromatography were optimized based on previous studies. The LC–MS analysis was conducted using an Agilent high-resolution Q-TOF system series 6520 equipped with an electrospray ionization source (ESI) and UHPLC system series 1290. For the analysis, a Kinetex-C18 (2.1 mm × 50 mm, dp = 1.7 μm) reversed-phase chromatographic column with a C18 precolumn guard (Phenomenex, Torrance, CA, USA) was employed. The MassHunter workstation software version B.06.00 (Agilent Technologies, Santa Clara, CA, USA) was used to control of the UHPLC-HRMS system, manage data acquisition, and perform qualitative and quantitative analysis. To ensure accuracy in mass measurements, a reference mass correction was implemented by utilizing lock masses of 121.050873 and 922.009798 (API-TOF Reference Mass Solution Kit; Agilent Technologies, Santa Clara, CA, USA). The process of optimizing instrument conditions commenced with the tunning of the MS detector in a positive mode operating within an extended dynamic range (2 GHz). Detailed information regarding the chromatographic and spectrometric parameters can be found in Table S1 .
6
HPLC-MS Metabolite Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following reagents were used: acetonitrile (HPLC-MS grade, Sigma-Aldrich, Taufkirchen, Germany), formic acid (MS grade, Sigma-Aldrich, Steinheim, Germany), L-methionine sulfone (Sigma-Aldrich, Taufkirchen, Germany), sodium hydroxide (Panreac, Montcada I Reixac, Spain) and ammonia (Panreac, Castellar del Vallès, Barcelona, Spain). For reference masses, purine and hexakis(1H,1H,3H-tetrafluoropropoxy)phosphazine (HP) from Agilent (API-TOF reference mass solution kit) were used. All solutions were prepared using MilliQ® water (Millipore, Billerica, MA, USA).
7
Mass Spectrometry Protocol for Metabolite Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Purified water was obtained using the Milli-Q Integral 3 system (Millipore SAS, Molsheim, France). Zomepirac sodium salt (used as an internal standard, IS), L-serine, L-arginine hydrochloride monohydrate, ascorbic acid, L-homoarginine hydrochloride, LC-MS grade methanol acetonitrile, formic acid and LC grade ethanol were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Pure p. a. ammonium solution (25%) was purchased from Avantor Performance Materials (Gliwice, Poland). The API-TOF reference mass solution kit (G1969–850001) and tuning solutions, ESI-L low concentration tuning mix (G1969–85000) and ESI-TOF Biopolymer Analysis reference masses (G1969–850003) were purchased from Agilent Technologies (Santa Clara, California, United States).
8
Mass Spectrometry Protocol Optimization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Purified water was obtained using the Milli-Q Integral 3 system (Millipore SAS, Molsheim, France). Zomepirac sodium salt (used as internal standard, IS), LS-MS-grade acetonitrile (ACN), methanol (MeOH), and formic acid (FA) were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Pure p.a. ammonium solution (25%) was purchased from Avantor Performance Materials (Gliwice, Poland). The API-TOF reference mass solution kit (G1969-850001) and tuning solutions, ESI-L low concentration tuning mix (G1969-85000), and ESI-TOF Biopolymer Analysis reference masses (G1969-850003) were purchased from Agilent Technologies (Santa Clara, CA, USA). Standards to confirm the identity of metabolites were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany) (taurine, pipecolic acid, leucine, histidine, phenylalanine, 3-methylhistidine, citric acid, tryptophan, adenosine, and stearoylcarnitine) and Avanti Polar Lipids Inc. (AL, USA) (LPI 18:0 and LPG 18:1).
9
UPLC-QTOF-MS for Metabolite Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The UPLC-QTOF-MS system used in this work consisted of an Agilent 1290 Infinity UPLC system coupled with an Agilent 6540 QTOF mass spectrometer. The UPLC system included a solvent reservoir, a degasser, a G4220A binary pump, a G1330B thermostat, a G4226A autosampler, a G1316C thermostatted column compartment, and a G4212A diode array detector. The mass spectrometer was equipped with an Agilent jet stream ESI probe.
Liquid chromatographic separation was achieved using gradient elution on a Waters ACQUITY UPLC BEH C18 (
GHz). The MS scan range was 50-1000 m/z at a scan rate 5 spectra/s. The MS/MS scan range was 50-1000 m/z at a scan rate of 3 spectra/s with an isolation width at narrow (~1.3 m/z). To maintain the mass accuracy, the mass spectrometer was calibrated and tuned before analysis, and internal reference masses from the reference mass solution were used for real-time mass correction at m/z 121.0508 and 922.0098 for the positive ion mode, and m/z 112.9885 and 1033.9881 for the negative ion mode throughout the acquisition process. The reference mass solution was prepared using an Agilent API-TOF reference mass solution kit (Part No: G1969-85001).
Liquid chromatographic separation was achieved using gradient elution on a Waters ACQUITY UPLC BEH C18 (
GHz). The MS scan range was 50-1000 m/z at a scan rate 5 spectra/s. The MS/MS scan range was 50-1000 m/z at a scan rate of 3 spectra/s with an isolation width at narrow (~1.3 m/z). To maintain the mass accuracy, the mass spectrometer was calibrated and tuned before analysis, and internal reference masses from the reference mass solution were used for real-time mass correction at m/z 121.0508 and 922.0098 for the positive ion mode, and m/z 112.9885 and 1033.9881 for the negative ion mode throughout the acquisition process. The reference mass solution was prepared using an Agilent API-TOF reference mass solution kit (Part No: G1969-85001).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!