Ripm 1640 medium

Manufactured by Thermo Fisher Scientific

Sourced in United States

RIPM-1640 medium is a cell culture medium formulated for the maintenance and growth of various cell lines. It is a complete, serum-free medium that supports the proliferation of a wide range of cell types without the need for additional supplementation. The medium is optimized to provide the necessary nutrients and growth factors for efficient cell culture.

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Lab products found in correlation

Insulin, by Beyotime (1 mentions) Epidermal growth factor (egf), by Beyotime (1 mentions) Basic fibroblast growth factor (bfgf), by Beyotime (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Mkn45, by Thermo Fisher Scientific (1 mentions) Hgc 27, by Thermo Fisher Scientific (1 mentions) Dmem f12 medium, by Thermo Fisher Scientific (1 mentions) Mgc 803, by Thermo Fisher Scientific (1 mentions) Leibovitz s l 15 medium, by Merck Group (1 mentions) Mda mb 435, by American Type Culture Collection (1 mentions) Hgc 27, by American Type Culture Collection (1 mentions) Mkn45, by American Type Culture Collection (1 mentions) Mgc 803, by American Type Culture Collection (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Sartorius (1 mentions) Dulbecco s modified eagle s medium dmem, by Boster Bio (1 mentions)

3 protocols using ripm 1640 medium

Culturing Melanoma and Gastric Cancer Cells

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Melanoma cell lines (MDA-MB-435 and A375) and gastric cancer cell lines (HGC-27, MKN-45, and MGC-803) were purchased from the American Type Culture Collection. MDA-MB-435 non-stem cells were cultured in Leibovitz's L-15 medium (MilliporeSigma, USA) supplemented with 10% fetal bovine serum (FBS). A375 and MGC-803 non-stem cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, USA) supplemented with 10% FBS. HGC-27 and MKN-45 non-stem cells were cultured in RIPM-1640 medium (Gibco) supplemented with 10% FBS. Melanoma stem cells and gastric cancer stem cells were cultured in DMEM/F12 medium (Invitrogen, USA) supplemented with 10 ng/mL basic fibroblast growth factor (Beyotime Biotechnology, Shanghai, China), 20 ng/mL epidermal growth factor (Beyotime), 5 μg/mL insulin (Beyotime), and 2% B-27 (MilliporeSigma). Melanoma stem cells, gastric cancer stem cells, gastric non-stem cells, and A375 non-stem cells were cultured at 37°C in a humidified atmosphere with 5% CO₂. MDA-MB-435 non-stem cells were cultured in a 100% humidified atmosphere at 37°C.

Zhang Y., Yang X., Cui Y, & Zhang X. (2021). Suppression of RNA editing by miR-17 inhibits the stemness of melanoma stem cells. Molecular Therapy. Nucleic Acids, 27, 439-455.

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Trichinella spiralis Muscle Larvae Isolation

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The T. spiralis (ISS 534 strain) used in this study was maintained in female Wistar rats in our laboratory (3500 ML/rat, maintained for about 6 weeks after oral infection), and T. spiralis muscle larvae were recovered from the muscles of infected rats with a standard pepsin/hydrochloric acid digestion method [17 (link)]. After washing with normal saline containing 2 × penicillin-streptomycin (Biological Industries, Kibbutz Beit Haemek, Israel), 5000 muscle larvae/ml were cultured in RIPM1640 medium (Gibco, California, USA) containing 2 × penicillin-streptomycin at a 37 °C for 18 h [5 (link)].

Li C., Li C., Xu F., Wang H., Jin X., Zhang Y., Liu X., Wang R., You X., Liu M., Bai X, & Yang Y. (2023). Identification of antigens in the Trichinella spiralis extracellular vesicles for serological detection of early stage infection in swine. Parasites & Vectors, 16, 387.

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Inflammatory Cell Models for Bioactive Equivalence

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Lipopolysaccharide (LPS)-stimulated NCM460 damage (Bhattacharyya et al., 2008 (link)) and LPS-stimulated THP-1-derived macrophage inflammation (Perezperez et al., 1995 (link)) were used as two cell models to assess the bioactive equivalence between components combination and HQD. NCM460 and THP-1 were obtained from Model Animal Research Center of Nanjing University and Stem Cell Bank of Chinese Academy of Sciences, respectively. Cells were grown at 37°C under a humidified atmosphere with 5% (v/v) CO₂. NCM460 and THP-1 were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Boster Biological Technology Co., Ltd.) and Roswell Park Memorial Institute (RIPM) 1640 medium (Gibco-Thermo Fisher Scientific, United States) respectively, containing 10% fetal bovine serum and 1% penicillin-streptomycin (Biological Industries, Israel).

Dai X.M., Cui D.N., Wang J., Zhang W., Zhang Z.J, & Xu F.G. (2018). Systems Pharmacology Based Strategy for Q-Markers Discovery of HuangQin Decoction to Attenuate Intestinal Damage. Frontiers in Pharmacology, 9, 236.

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