The pcDNA-Capn4 and pcDNA-control were purchased from Thermo Fisher Scientific. We purchased miR-124 mimics, anti-miR-124, and miR-control from GenePharma (Shanghai, China). Cell transfection was performed using Lipofectamine 2000 (Thermo Fisher Scientific).
Anti mir 124
Manufactured by GenePharma
Sourced in China
Anti-miR-124 is a laboratory reagent used for the study of microRNA-124. It is designed to inhibit the function of microRNA-124 in experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions)
Mir control, by GenePharma (1 mentions)
Mir 124 mimic, by GenePharma (1 mentions)
Pcdna control, by Thermo Fisher Scientific (1 mentions)
Si pvt1, by GenePharma (1 mentions)
Pyrrolidinedithio carbamate ammonium pdtc, by Merck Group (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Pcdna3.1 vector, by Thermo Fisher Scientific (1 mentions)
Mir nc, by GenePharma (1 mentions)
Anti mir nc, by GenePharma (1 mentions)
Mir con, by GenePharma (1 mentions)
Anti mir con, by GenePharma (1 mentions)
Anti mir con, by Thermo Fisher Scientific (1 mentions)
3 protocols using anti mir 124
1
Capn4 Overexpression and miR-124 Regulation
2
Modulating PVT1 and miR-124 in HK-2 cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pyrrolidinedithio-carbamate ammonium (PDTC) was purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA). Small interference RNA targeting PVT1 (si-PVT1) and its scramble control (si-NC), miR-124 mimic (miR-124) and its negative control (miR-NC), miR-124 inhibitor (anti-miR-124) and its negative control (anti-miR-NC) were purchased from GenePharma Co. Ltd. (Shanghai, China). Also, PVT1 overexpression plasmid was generated by subcloning PVT1 full-length fragments into pcDNA3.1 vector (Invitrogen, Carlsbad, CA, USA). All these substances were transfected into HK-2 cells using Lipofectamine 2000 reagent (Invitrogen).
3
Modulation of miR-124 Expression in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MiR-124 mimics, miRNA mimic negative control (miRcon), miR-124 inhibitors (anti-miR-124), and miRNA inhibitor negative control (anti-miR-con) were purchased from GenePharma (Shanghai, China). Synthetic miR-124 mimics, miR-con, miR-124 inhibitors, and anti-miR-con were transfected into cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. Small interfering RNA (siRNA) vectors targeting BACE1 were purchased from GenePharma (Shanghai, China). Transfection was conducted using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!