Pcb118

Manufactured by AccuStandard

Sourced in United States

PCB118 is a certified reference standard for polychlorinated biphenyl (PCB) compounds. It is used for the identification and quantification of PCB118 in various environmental and analytical applications.

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Lab products found in correlation

Pcb153, by AccuStandard (2 mentions) Fetal bovine serum (fbs), by Cell Signaling Technology (1 mentions) Anti il 1β antibody, by Abcam (1 mentions) Gapdh polyclonal antibody, by Bioworld Technology (1 mentions) Pcb 126, by AccuStandard (1 mentions) Newborn calf serum, by Thermo Fisher Scientific (1 mentions) Somatostatin, by Merck Group (1 mentions) Bovine insulin, by Merck Group (1 mentions) Coon s modified ham s f 12 medium, by Merck Group (1 mentions) Glycyl l histidyl l lysine acetate, by Merck Group (1 mentions) Bovine tsh, by Merck Group (1 mentions) Hydrocortisone, by Merck Group (1 mentions) Transferrin, by Merck Group (1 mentions)

5 protocols using pcb118

Islet β-TC-6 Cells Inflammatory Response

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Mouse islet β-TC-6 cells were purchased from TONGPAI (Shanghai) Biotechnology Co. Ltd. PCB118 was purchased from AccuStandard Inc. (New Haven, CT, USA). Cell Counting Kit-8 (CCK8), ROS detection kit, and DMSO were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Specific antibodies against NLRP3, caspase-1, and fetal bovine serum were purchased from Cell Signaling Technology (CST, Boston, MA, USA). Anti-caspase-1 antibody (EPR4321) and anti-IL-1 β antibody were purchased from Abcam (USA). GAPDH polyclonal antibody was purchased from BioWorld (USA). ELISA kits for IL-6, IL-18, and CCL-2 were purchased from Cheng Lin Biological (Beijing, China).

Jiang C., Wang Y., Guo M., Long Y., Chen J., Fan F., Tang S, & Xu Y. (2021). PCB118 Induces Inflammation of Islet Beta Cells via Activating ROS-NLRP3 Inflammasome Signaling. BioMed Research International, 2021, 5522578.

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Quantification of PCB118 and PCB153

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PCB118 and PCB153 were purchased from AccuStandard Inc. (New Haven, USA).

Mesnier A., Champion S., Louis L., Sauzet C., May P., Portugal H., Benbrahim K., Abraldes J., Alessi M.C., Amiot-Carlin M.J., Peiretti F., Piccerelle P., Nalbone G, & Villard P.H. (2015). The Transcriptional Effects of PCB118 and PCB153 on the Liver, Adipose Tissue, Muscle and Colon of Mice: Highlighting of Glut4 and Lipin1 as Main Target Genes for PCB Induced Metabolic Disorders. PLoS ONE, 10(6), e0128847.

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Investigating Chlorinated Biphenyl Toxicity

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3,3’,4,4’,5-pentachlorobiphenyl (PCB 126), 2,3’,4,4’,5-pentachlorobiphenyl (PCB 118), and 2,2’,4,4’,5,5’-hexachlorobiphenyl (PCB 153) were purchased from AccuStandard Inc. (New Haven, CT, USA). Phorbol 12-myristate 13-acetate (PMA), dimethyl sulfoxide (DMSO), lipopolysaccharide (LPS), BMS-345541 (NF-κB antagonist) were purchased from Sigma (St. Louis, MO, USA). Recombinant human interferonγ (IFN-γ) was purchased from PeproTech (Rocky Hill, NJ, USA). CH-223191(AhR antagonist) was purchased from BioVision (Milpitas, CA, USA)

Wang C., Petriello M.C., Zhu B, & Hennig B. (2019). PCB 126 Induces Monocyte/Macrophage Polarization and Inflammation through AhR and NF-κB pathways. Toxicology and applied pharmacology, 367, 71-81.

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FRTL-5 Cell Culture Protocol

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FRTL-5 cells were kindly provided by Dr. Zheng Xuqin (Department of Endocrinology, First Affiliated Hospital of Nanjing Medical University) [28 (link)], who was kindly gifted these cells by Professor Michael Derwahl (Department of Medicine, St. Hedwig Hospital) [29 (link)]. Coon’s modified Ham’s F-12 medium, transferrin, bovine insulin, hydrocortisone, somatostatin, glycyl-L-histidyl-L-lysine acetate, and bovine TSH were purchased from Sigma-Aldrich (USA). Newborn calf serum was purchased from Gibco (USA). Penicillin and streptomycin were purchased from HyClone (USA). PCB118 (purity: 100%, analyzed using GC/MS) was purchased from AccuStandard (USA) and was dissolved initially in 612 μL dimethyl sulfoxide (DMSO) and maintained as a 25 mM stock solution in the dark. When needed, this solution was diluted to the required concentration with culture media. The final concentration of DMSO in culture medium was maintained below 0.1%.

Yang H., Chen H., Guo H., Li W., Tang J., Xu B., Sun M., Ding G., Jiang L., Cui D., Zheng X, & Duan Y. (2015). Molecular Mechanisms of 2, 3′, 4, 4′, 5-Pentachlorobiphenyl-Induced Thyroid Dysfunction in FRTL-5 Cells. PLoS ONE, 10(3), e0120133.

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Quantifying Oxidative Stress Biomarkers

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The PCB118 was purchased from AccuStandard, Inc (New Haven, Connecticut). The 2′,7′-dichlorofluorescein diacetate (DCFH-DA), 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), and dimethyl sulfoxide (DMSO) were purchased from Sigma Chemical Corp (St Louis, Missouri). The Dulbecco’s modified Eagle’s medium (DMEM) and Fetal Bovine Serum (FBS) were obtained from Gibco and Life Technologies (Grand Island, New York). The kits for the detection of glutathione peroxidase (GSH-Px) activity, MDA content, and SOD activity were purchased from Nanjing Jiancheng Biological Corp (New Jersey). All reagents used were of analytical grade. The PCB118 stock solution (20 µg/mL) was prepared in DMSO and further diluted to working concentrations. The concentration of DMSO in the culture medium was lower than 0.2% (vol/vol).

Hashmi M.Z., Hasnain A., Syed J.H., Tariq M., Su X., Mubarak H., Nasim W, & Shen C. (2018). PCB118-Induced Cell Proliferation Mediated by Oxidative Stress and MAPK Signaling Pathway in HELF Cells. Dose-Response, 16(1), 1559325817751525.

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