Human inflammatory cytokine citometric bead array kit

Hematological parameters (leukocyte and neutrophil count, hemoglobin and hematocrit) and C-reactive protein were performed in Clinical Laboratory of Dante Pazzanese Institute of Cardiology, with routine automated methodology (cytochemical/isovolumetric and kinetic assay, respectively) immediately after collection. The plasma levels of IL-6, IL-1β, IL-10, IL-8, IL-12p70 and TNF-α were determined using BD ^™ Human Inflammatory Cytokine Citometric Bead Array Kit and BD Accuri cytometer according to manufacturer's instructions (BD Biosciences, San Jose, CA, USA).

The biochemical parameters were evaluated with routine automated methodology in Clinical Laboratory of Dante Pazzanese Institute of Cardiology immediately after blood collection (22 ml). Alanine transaminase, aspartate transaminase, lactic dehydrogenase and creatine kinase were determined by kinetic assay; Troponin (I), proBNP, myoglobin, and creatine kinase-MB were evaluated by chemiluminescence assay.
The serum levels of IL-6, IL-1β, IL-10, IL-8, IL-12p70 and TNF-α were determined using BD^™ Human Inflammatory Cytokine Citometric Bead Array Kit and BD Accuri cytometer according to manufacturer’s instructions (BD Biosciences, San Jose, CA, USA). The detection limit was 3.6 pg/ml for IL-8; 7.2 pg/ml for IL-1β; 2.6 pg/ml for IL-6; 3.3 pg/ml for IL-10 of; 3.7 pg/ml for TNF-α, and 1.9 pg/ml for IL12p70.