Cryopreserved human hepatocytes, HLM, thawing and incubation media were
purchased from BioreclamationIVT (Baltimore, MD, USA). NADPH regenerating system
solutions A and B were purchased from Corning, Inc. (part #s 451220 and
451200, respectively). AH-7921 (97.94±1.04% purity) was kindly
provided by the Drug Enforcement Administration Special Testing and Research
Laboratory (Dulles, VA, USA). Liquid chromatography-mass spectrometry
(LC-MS)grade acetonitrile and Trypan Blue were purchased from Sigma-Aldrich (St.
Louis, MO, USA), while potassium phosphate, glacial acetic acid and formic acid
(LC-MS grade) were obtained from Fisher Scientific (Waltham, MA, USA). APCI
positive calibration solution was from SCIEX (part # 4460131, Redwood
City, CA, USA). Water was purified in house with an ELGA Purelab Ultra Analytic
purifier (Siemens Water Technologies, Lowell, MA, USA).
purchased from BioreclamationIVT (Baltimore, MD, USA). NADPH regenerating system
solutions A and B were purchased from Corning, Inc. (part #s 451220 and
451200, respectively). AH-7921 (97.94±1.04% purity) was kindly
provided by the Drug Enforcement Administration Special Testing and Research
Laboratory (Dulles, VA, USA). Liquid chromatography-mass spectrometry
(LC-MS)grade acetonitrile and Trypan Blue were purchased from Sigma-Aldrich (St.
Louis, MO, USA), while potassium phosphate, glacial acetic acid and formic acid
(LC-MS grade) were obtained from Fisher Scientific (Waltham, MA, USA). APCI
positive calibration solution was from SCIEX (part # 4460131, Redwood
City, CA, USA). Water was purified in house with an ELGA Purelab Ultra Analytic
purifier (Siemens Water Technologies, Lowell, MA, USA).