Fetal bovine serum (FBS) and RPIM‐1640 medium (Gibco, Grand Island, NY, USA); 5‐FU, 6‐MP, 6‐TG, DXR, VCR, HU, cisplatin and PRPP (Sigma‐Aldrich, St. Louis, USA); Annexin V‐APC/PI Apoptosis Detection Kit (MultiSciences, Hangzhou, China); FuGENE‐6 and CellTiter‐Glo Luminescent kit (Promega, Madison, WI, USA); Amicon purification column‐100kD (Millipore, Burlington, MA, USA); tissue culture plate (Corning, NY, USA); IRDye800COR‐lgG second antibody (LI‐COR, Lincoln, Nebraska, USA); nitrocellulose membrane 0.45 μm (GE Healthcare, Chicago, IL, USA); [U‐13C6]‐d ‐glucose (Cambridge Isotope Laboratories, Andover, MA, USA, cat. No. CLM‐1396‐1).
Annexin 5 apc pi apoptosis detection kit
Manufactured by MultiSciences Biotech
Sourced in China
The Annexin V-APC/PI Apoptosis Detection Kit is a laboratory product designed to detect and analyze apoptosis, a form of programmed cell death, in cell samples. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide (PI), a dye that binds to DNA, to differentiate between viable, early apoptotic, and late apoptotic/necrotic cells.
Automatically generated - may contain errors
Lab products found in correlation
Propidium iodide staining solution, by MultiSciences Biotech (2 mentions)
Facscan, by BD (2 mentions)
Cell cycle detection kit, by MultiSciences Biotech (2 mentions)
Tissue culture plate, by Corning (1 mentions)
0.45 μm nitrocellulose membrane, by GE Healthcare (1 mentions)
U 13c6 d glucose, by Cambridge Isotopes (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Facscalibur, by BD (1 mentions)
Fluorescence microscope, by Nikon (1 mentions)
Hoechst 33258, by Beyotime (1 mentions)
Facsaria 3 cell sorter, by BD (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by MultiSciences Biotech (1 mentions)
10 protocols using annexin 5 apc pi apoptosis detection kit
1
Cytotoxicity Evaluation of Chemotherapeutics
2
Annexin V-APC/PI Apoptosis Assay
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We conducted the apoptosis assay using an Annexin V-APC/PI Apoptosis Detection Kit (Multisciences, China). The obtained data were analyzed with flow cytometry (BD Biosciences, USA). The total apoptosis rate was calculated based on the ratio of early and late apoptotic cells.
3
Apoptosis Analysis by Hoechst and Flow Cytometry
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Hoechst staining and flow cytometry analysis were applied to measured apoptosis. Cells were incubated with Hoechst 33258 (Beyotime Biotech, Nanjing, China) for 1 h after fixed with 4% paraformaldehyde. The apoptotic cells were observed by fluorescence microscope (Nikon, Japan). Apoptotic cells were also detected with Annexin V-APC/PI apoptosis detection kit (MULTI SCIENCES, Hangzhou, China). Briefly, cells were trypsinized and harvested after appropriate treatment. Harvested cells were then incubated with Annexin V-APC and propidium iodide (PI) for 15 min in the dark according to the manufacturer’s instructions. The stained cells were determined by flow cytometer (FACSCalibur, BD, United States).
4
Cell Cycle and Apoptosis Assay
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The transfected cells were collected, washed carefully with phosphate-buffered saline (PBS), fixed with 75% ethanol, stored at − 20 °C overnight, incubated with RNAse and stained with propidium iodide staining solution (MultiSciences, Hangzhou, China) at RT for 15 min for the cell cycle analysis.
The apoptotic assay was conducted using an Annexin V-APC/PI Apoptosis Detection Kit (Multisciences, Hangzhou, China) and analyzed with a flow cytometry (FACScan, BD Biosciences). The ratio of early and terminal apoptotic cells was detected to calculate the apoptotic rate.
The apoptotic assay was conducted using an Annexin V-APC/PI Apoptosis Detection Kit (Multisciences, Hangzhou, China) and analyzed with a flow cytometry (FACScan, BD Biosciences). The ratio of early and terminal apoptotic cells was detected to calculate the apoptotic rate.
5
Investigating Cell Cycle and Apoptosis
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MDA-MB-231/alisertib cells treated differently were washed with PBS. The cells were then fixed with 75% ethanol and kept at −20°C overnight. The cell cycle detection kit (MultiSciences, Hangzhou, China) and Annexin V-APC/PI Apoptosis Detection Kit (MultiSciences, China) were used respectively for cell cycle distribution and apoptotic cell staining. The different cells were ultimately analyzed by flow cytometry.
6
Apoptosis Quantification by Flow Cytometry
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The apoptotic assay was conducted using an Annexin V-APC/PI Apoptosis Detection Kit (Multisciences, China) and analyzed with a flow cytometry (BD Biosciences, USA). The ratio of early and late apoptotic cells was detected to calculate the apoptotic rate.
7
Apoptosis and Cell Cycle Analysis
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Apoptosis was measured by the flow cytometric detection (FACScan, BD Biosciences) of phosphatidylserine externalization using Annexin V‐APC/PI Apoptosis Detection Kit (Multiscience, Hangzhou, China). Cells were discriminated into viable cells, dead cells, early apoptotic cells, and late apoptotic cells, and then, the relative ratio of early and late apoptotic cells accounting for total cells was compared from each treated group.
Cells for cell cycle analysis were stained with propidium iodide staining solution (Multisciences, Hangzhou, China) following the protocol and analyzed by the flow cytometer mentioned above. The percentage of the cells in G0‐G1, S, and G2‐M phases were counted and compared.
Cells for cell cycle analysis were stained with propidium iodide staining solution (Multisciences, Hangzhou, China) following the protocol and analyzed by the flow cytometer mentioned above. The percentage of the cells in G0‐G1, S, and G2‐M phases were counted and compared.
8
Cell Cycle and Apoptosis Analysis
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HUVECs treated differently were harvested and carefully washed with PBS. Then, 75% ethanol was used to fix the cells and stored at −20 °C overnight. Afterwards, cell cycle distribution was detected by cell cycle detection kit (Multisciences, China). The Annexin V-APC/PI Apoptosis Detection Kit (Multisciences, China) was used to stain the apoptotic cells according to the manufacturer’s instructions. Cell cycle arrest and apoptosis were analyzed by flow cytometry after reagent pretreatment.
9
Annexin V-FITC/PI Apoptosis Assay
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Apoptosis was detected using an annexin V-FITC/PI apoptosis detection kit or annexin V-APC/PI apoptosis detection kit according to the manufacturer's instructions [MultiSciences (Lianke) Biotech Co., Ltd.]. Briefly, VSMCs were plated into six-well plates and incubated with drugs for optimal intervals using the results from MTT assay. The cells were harvested, washed twice with pre-chilled PBS, and centrifuged (1,000 x g for 5 min at 4˚C), followed by staining with annexin V-FITC/PI or annexin V-APC/PI at 4˚C for 10 min in the dark and then stained cells were analyzed by flow cytometry (BD FACSAria™ III Cell Sorter; cat. no. 648282; BD Biosciences). Flow cytometry data analysis of the proportion of apoptotic cells was performed using the FlowJo 7.6.1 software (Flowjo LLC).
10
Annexin-V-APC/PI Apoptosis Detection
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Apoptosis was detected by staining with the Annexin‐V‐APC/PI Apoptosis Detection Kit (MultiSciences). Analysis was performed by flow cytometry using the manufacturer's protocol.29 , 30
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