Cell density was monitored during the experiments to obtain growth curves. Briefly, 2 mL subsamples from each flask were collected and fixed with Lugol’s iodine solution (1.5% v/v). One milliliter of this solution was used to fill a Sedgewick Rafter counting cell chamber and cell counts were performed by using a Zeiss Axioskop 2 Plus light microscope (Carl Zeiss, Göttingen, Germany).
Axioskop 2 plus light
Manufactured by Zeiss
Sourced in Germany
The Zeiss Axioskop 2 Plus is a light microscope designed for a wide range of applications in laboratory and research settings. It features high-quality optics and illumination systems to provide clear, detailed images of specimen samples.
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Lab products found in correlation
2 protocols using axioskop 2 plus light
1
Cell Density Monitoring Protocol
2
Measuring Microalgae Cell Density
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To assess cell density, 2 mL of cell suspension were collected from each tank and fixed with Lugol’s iodine solution (1.5% v/v). Then, 1 mL of this solution was used to fill a Sedgewick Rafter counting cell chamber. Cells were then counted using a Zeiss Axioskop 2 Plus light microscope (Carl Zeiss, Göttingen, Germany).
The growth rate was estimated from cell concentration measurements using the following equation:
where µ is the growth rate, Cn−1 and Cn are cell concentrations (mL−1) at day n − 1 (tn−1) and day n (tn).
The growth rate was estimated from cell concentration measurements using the following equation:
where µ is the growth rate, Cn−1 and Cn are cell concentrations (mL−1) at day n − 1 (tn−1) and day n (tn).
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