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Silencer select predesigned sirnas

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Silencer Select predesigned siRNAs are synthetic small interfering RNA (siRNA) molecules designed to target and silence specific genes in cellular experiments. They are provided as a ready-to-use solution to facilitate gene knockdown studies.

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Lab products found in correlation

Human mb21d1 cgas sirnas, by Thermo Fisher Scientific (2 mentions) Lipofectamin 2000, by Thermo Fisher Scientific (2 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Non targeting sirna, by Thermo Fisher Scientific (1 mentions) Silencer select sirna, by Thermo Fisher Scientific (1 mentions) Lipod293 in vitro dna transfection reagent, by SignaGen (1 mentions) Rnaimax, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Silencer Select siRNAs (565 citations) Silencer Select (341 citations) SiRNAs (258 citations) Silencer siRNA (37 citations) Silencer Select Predesigned (5 citations) Predesigned siRNAs (2 citations) Ambion® Silencer® Select Predesigned siRNAs (2 citations) SAG siRNA (Silencer select predesigned siRNA) (2 citations)

5 protocols using silencer select predesigned sirnas

1

siRNA-mediated Silencing of SQR Gene

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SiRNA oligonucleotides, Silencer Select Pre‐designed siRNAs (Life Technologies, Carlsbad, CA, USA), were used for the transient silencing of SQR. For SQR, the applied sequence contained the 5′‐3′ sense, GCUCAGUAAACAUCCCGUUtt and antisense 3′‐5′, AACGGGAUGUUUACUGAGCca. This SQR‐silencing siRNA (Ambion s81773) was complementary with the mRNAs belonging to RefSeq NM_001162503.1 and NM_021507.5 genes and also targeted exon 3. For negative control, non‐targeting siRNAs were applied with the same chemical modifications for enhanced efficacy as in other Silencer Select siRNAs (Ambion). Silencing was conducted as described previously (Modis K, Faseb 2012). Cells (80,000 cells/well) were seeded into 6‐well tissue culture plates and cultured in normal culture medium to reach 50% confluence. At this point, the growth medium was replaced with Opti‐MEM medium lacking FBS and antibiotics/antimicotics, followed by transfection with 25 pmol siRNA fragments per well at 30 nM forming complexes with 7.5 ml of Lipofectamine® RNAiMAX (Life Technologies). Control cells were transfected in parallel with non‐targeting siRNA (Life Technologies).
Luna‐Sánchez M., Hidalgo‐Gutiérrez A., Hildebrandt T.M., Chaves‐Serrano J., Barriocanal‐Casado E., Santos‐Fandila Á., Romero M., Sayed R.K., Duarte J., Prokisch H., Schuelke M., Distelmaier F., Escames G., Acuña‐Castroviejo D, & López L.C. (2016). CoQ deficiency causes disruption of mitochondrial sulfide oxidation, a new pathomechanism associated with this syndrome. EMBO Molecular Medicine, 9(1), 78-95.
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2

Plasmid Transfection and RNA Interference

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Plasmid transfections (vector, HA-RNMT, FLAG-RAM, CBP-MYC) were performed using LipoD293™ In Vitro DNA Transfection Reagent per protocol (SignaGen). Cells were harvested for protein or RNA 24 h post-transfection. Silencer Select Pre-designed siRNAs were obtained from Life Technologies and transfected using RNAiMax (Invitrogen; 20nM). Cells were harvested for protein or RNA 48 h post-transfection. Silencer Select negative control #2 (Life Technologies) was used as a transfection control.
Posternak V., Ung M.H., Cheng C, & Cole M.D. (2016). MYC Mediates mRNA Cap Methylation of Canonical Wnt/β-catenin Signaling Transcripts by Recruiting CDK7 and RNA Methyltransferase. Molecular cancer research : MCR, 15(2), 213-224.
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3

Transfection of siRNAs in Cells

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Cells were transfected with siRNAs using Lipofectamin 2000 reagent (Thermo Fisher Scientific) according to the manufacurer’s instruction. Silencer select predesigned siRNAs were purchased from Life Technologies. Sequence of mmLmnB1 siRNA Nr. 2: sense: 5’ CAUCAGUCAGUUACAAAUATT 3’; antisense: 5’ UAUUUGUAACUGACUGAUGTG 3’. mmLmnB1 siRNA Nr. 1: sense: 5’ GGAAGUUUAUUCGCUUGAAtt 3’; antisense: 5’ UUCAAGCGAAUAAACUUCCca 3’. Human MB21D1 (cGAS) siRNAs were purchased from ThermoFisher Scientific (Cat: AM16708, sicGAS 1 ID: 129125, sicGAS 2 ID: 129126).
Glück S., Guey B., Gulen M.F., Wolter K., Kang T.W., Schmacke N.A., Bridgeman A., Rehwinkel J., Zender L, & Ablasser A. (2017). Innate immune sensing of cytosolic chromatin fragments through cGAS promotes senescence. Nature cell biology, 19(9), 1061-1070.
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4

Transfection of siRNAs in Cells

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Cells were transfected with siRNAs using Lipofectamin 2000 reagent (Thermo Fisher Scientific) according to the manufacurer’s instruction. Silencer select predesigned siRNAs were purchased from Life Technologies. Sequence of mmLmnB1 siRNA Nr. 2: sense: 5’ CAUCAGUCAGUUACAAAUATT 3’; antisense: 5’ UAUUUGUAACUGACUGAUGTG 3’. mmLmnB1 siRNA Nr. 1: sense: 5’ GGAAGUUUAUUCGCUUGAAtt 3’; antisense: 5’ UUCAAGCGAAUAAACUUCCca 3’. Human MB21D1 (cGAS) siRNAs were purchased from ThermoFisher Scientific (Cat: AM16708, sicGAS 1 ID: 129125, sicGAS 2 ID: 129126).
Glück S., Guey B., Gulen M.F., Wolter K., Kang T.W., Schmacke N.A., Bridgeman A., Rehwinkel J., Zender L, & Ablasser A. (2017). Innate immune sensing of cytosolic chromatin fragments through cGAS promotes senescence. Nature cell biology, 19(9), 1061-1070.
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5

ABCB1 Gene Silencing in NCI-H295R Cells

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A small interfering RNA (siRNA) duplex targeting regions of ABCB1 gene and a non-targeting negative control siRNA (si-control) were used (Silencer® Select Pre-Designed siRNAs, LifeTechnologies, Milan, Italy). The si-ABCB1 sense and anti-sense sequences were: 5′-CGAUACAUGG UUUCCGAUTT-3′ and 5′-AUCGGAAAACCAUGUAUCGGA-3' (si-ABCB1 10419). NCI-H295R cells were transfected with si-ABCB1 or si-control using the Lipofectamine RNAiMAX system (LifeTechnologies, Milan, Italy) according to the manifacturer's instructions.
Fragni M., Palma Lopez L.P., Rossini E., Abate A., Cosentini D., Salvi V., Vezzoli S., Poliani P.L., Bosisio D., Hantel C., Tiberio G.A.M., Grisanti S., Memo M., Terzolo M., Berruti A, & Sigala S. (2019). In vitro cytotoxicity of cabazitaxel in adrenocortical carcinoma cell lines and human adrenocortical carcinoma primary cell cultures☆. Molecular and cellular endocrinology, 498.
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