Anti itgb1 antibody

Paraffin sections were dewaxed, and treated with sodium citrate antigen retrieval. Then tissue sections were blocked with 5% goat serum in PBST for 30 min at room temperature, and incubated with primary antibodies: anti-type I collagen antibody (1:200, Abcam, UK), anti-ITGB1 antibody (1:300, Abcam, UK) and anti-NF-κB (1:500, Abcam, UK) for overnight at 4 °C. For immunohistochemistry, samples were then incubated with goat anti-rabbit secondary antibodies (Abcam, UK) and stained with DAPI (Solarbio, China). For immunofluorescence, samples were then incubated with HRP labeled goat anti-rabbit secondary antibodies (Abcam, UK) and stained with hematoxylin (Solarbio, China). The intensity of protein expression was analyzed by image pro plus 6.0 software (USA).

To explore the mechanism underlying the regulatory effects of PLA2R1 on thyroid cancer, RNA sequencing was performed to identify DEGs between normal thyroid tissues or cells and thyroid cancer tissues or cells. The selected DEGs were entered into the Gene Expression Profiling Interactive Analysis (GEPIA) database to verify the correlations between the corresponding proteins.
The proteins encoded by the selected DEGs were analyzed by a Co-IP experiment. Cell lysates were incubated with an anti-ITGB1 antibody (Abcam, Cambridge, UK) or control IgG (Cell Signaling Technology, BSN, Danvers, MA, USA) for 2 h, and 20 μL of Protein A/G PLUS-Agarose beads was then added and incubated overnight at 4 °C. The antibody-antigen conjugates were then washed with 500 μL of complete Co-IP/wash buffer, and the proteins were eluted in Laemmli sample buffer and denatured at 68 °C for 10 min. The eluates were analyzed by Western blotting.