S6883

Manufactured by Merck Group

Sourced in United States, Germany

S6883 is a laboratory equipment product. It is designed for general laboratory applications. The core function of this product is to provide a reliable and versatile tool for researchers and scientists working in various laboratory settings.

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Lab products found in correlation

8 br camp, by Merck Group (1 mentions) V6130, by Merck Group (1 mentions) Pacap, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) C4382, by Merck Group (1 mentions) L 733 060, by Merck Group (1 mentions) L 733 060, by Bio-Techne (1 mentions) M3262, by Merck Group (1 mentions) Sc 14104, by Santa Cruz Biotechnology (1 mentions)

5 protocols using s6883

Detailed Protocol for Peptide Preparation

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All reagents were obtained from Sigma Aldrich (Poole, Dorset, UK) or Fisher Scientific (Loughborough, UK). 8-bromo-cAMP (8-Br-cAMP, B5386, Sigma) was prepared fresh immediately before use. Pituitary adenylate cyclase-activating polypeptide-38 (PACAP, A1439, Sigma), the dominant peptide in all tissues examined, was used in these experiments. Vasoactive intestinal peptide (VIP, V6130, Sigma), PACAP, corticotrophin-releasing hormone (CRH, C3042, Sigma) and calcitonin gene-related peptide (CGRP, C0168, Sigma) were dissolved in 175 mM acetic acid and substance P (S6883, Sigma) in 50 mM acetic acid with 1% (w/v) bovine serum albumin (A8806, Sigma). Tetrodotoxin (TTX, NA-120, Enzo Life Sciences, Exeter, UK), dissolved at 1 mg/ml in 1% (v/v) acetic acid, was stored at −20°C and thawed immediately before use. Peptides were stored in aliquots at −80°C and defrosted immediately before use.

Moran A.W., Al-Rammahi M.A., Batchelor D.J., Bravo D.M, & Shirazi-Beechey S.P. (2018). Glucagon-Like Peptide-2 and the Enteric Nervous System Are Components of Cell-Cell Communication Pathway Regulating Intestinal Na+/Glucose Co-transport. Frontiers in Nutrition, 5, 101.

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Exploring Nociceptive Modulation via NK1R

Cited 1 time

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The drugs used were (i) the NK1R agonist, Substance P (SP; 1 and 2 μg/μl; #S6883, Sigma, USA), (ii) the NK1R antagonist, L-733,060 (1 ×: 0.0176 µg/µl and × 0.176 µg/µl; #1145, Tocris Bioscience, UK) and (iii) the cholinergic receptor agonist, carbamoylcholine chloride (carbachol; 0.156 μg/μl; #C4382, Sigma, USA).
The functional effects of different doses of SP and L-733,060 were explored in this study. Notably, 1 × concentration of L-733,060 in the present study is anti-nociceptive on microinjection into the rostral ventral medulla^{48 (link)}. While we initially used the lower dose of L-733,060, arising from the results, especially in behaving animals, we also examined the effect of the higher dose of L-733,060 to explore whether the different aspects of nociception are differentially sensitive to antagonism by the antagonist. The concentration of carbachol used in the experiments is based on published work^{65 (link)}.

Ng S.Y., Ariffin M.Z, & Khanna S. (2021). Neurokinin receptor mechanisms in forebrain medial septum modulate nociception in the formalin model of inflammatory pain. Scientific Reports, 11, 24358.

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Intrathecal Activation of Spinal Nociception

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To permit direct activation of spinal nociceptive circuitry, animals received intrathecal delivery of the glutamate receptor agonist N-methyl-D-aspartate (NMDA) (M3262, Sigma Aldrich, St. Louis, MO) or the NK1 receptor agonist substance P (sP) (S6883, Sigma Aldrich, St. Louis, MO). Two days after BoNT-B or vehicle pre-treatment, anesthesia was induced and maintained with 2.5% isoflurane. Maintenance anesthesia was administered via a nosecone. Mice were then intrathecally (i.t.) injected between the L5 and L6 vertebrae with 5 μL of NMDA (0.25nM), sP (3.71 pM) or saline. A flick of the tail upon insertion of the needle was taken to indicate appropriate needle placement. Mice remained anesthetized and the spinal cords were harvested 5 min post-injection for western blot analysis of pAkt and pGluA1.

Sikandar S., Gustavsson Y., Marino M.J., Dickenson A.H., Yaksh T.L., Sorkin L.S, & Ramachandran R. (2016). Effects of intraplantar Botulinum toxin-B on carrageenan-induced changes in nociception and spinal phosphorylation of GluA1 and Akt. The European journal of neuroscience, 44(1), 1714-1722.

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Effects of Substance P on Osteoclast and Osteoblast Cultures

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For all experiments, BMM/osteoclasts and osteoblast-like cell cultures of Tac1−/− and also partly WT (Suppl. Files 2) mice were stimulated with 10⁻⁸ and 10⁻¹⁰ M of recombinant SP (#S6883, Sigma-Aldrich Chemie GmbH, Schnelldorf, Germany), either for the last 24 h of culture time or for entire culture period with every medium exchange (only Tac1−/− cultures) to mimic neurotransmitter milieu predominant in WT tissues.

Niedermair T., Schirner S., Seebröker R., Straub R.H, & Grässel S. (2018). Substance P modulates bone remodeling properties of murine osteoblasts and osteoclasts. Scientific Reports, 8, 9199.

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Detecting Substance P in Experimental and Clinical Studies

Cited 1 time

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An affinity purified goat polyclonal SP antibody was utlized. It was obtained from Santa Cruz Biotechnology (code: sc-14104) and was used at a dilution of 1:25-1:50. This antibody is raised against a peptide mapping within an internal region of preprotachykinin 1 of human origin and is recommended for detection of mature SP and all isoforms of the protachykinin 1 precursor of various species [mouse, rat and human origin]. According to “Human Protein Reference Database” and “BLAST-Basic Local Alignment Search Tool”, the protein sequence of rabbit protachykinin 1 shows at least 85% identity with human protachykinin 1.
A rat monoclonal SP antibody was also used. It was obtained from Biogenesis, Poole, UK (code: 8450-0505). It was used at a dilution of 1:50-1:100. The antibody is reported to recognize the COOH terminal end of SP and has been previously used for immunohistochemical detection of SP in experimental animals and man [5 (link),33 (link)].
Control stainings included the use of SP blocking substance (code: sc14104P; Santa Cruz) (20-50 μg/ml antiserum) and SP peptide (full length SP peptide; S6883; Sigma) (50 μg/ml antiserum). Ordinary stainings with the SP antisera were performed in parallel. Other control stainings conformed to stainings when the primary antibodies were excluded (buffer instead of the antibody).

Song Y., Stål P.S., Yu J.G., Lorentzon R., Backman C, & Forsgren S. (2014). Inhibitors of endopeptidase and angiotensin-converting enzyme lead to an amplification of the morphological changes and an upregulation of the substance P system in a muscle overuse model. BMC Musculoskeletal Disorders, 15, 126.

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