Ovarian cancer cells A2780 cells and their cisplatin-resistant derivatives (referred in this study as A2780-CR) were obtained from Sigma (St Louis, MO, United States) while SK-OV-3 cells were from ATCC (Manassas, VA, United States). Cells were cultured in RPMI 1640 media with 10% fetal bovine serum and 1% antibiotics in a 5% CO2-humidified atmosphere at 37°C. The si-HOTTIP as well as si-ZEB2 was purchased from Shanghai GenePharma Co., Ltd. (China).
Sizeb2
Manufactured by GenePharma
Sourced in China
SiZEB2 is a high-performance cell imaging system designed for advanced microscopy applications. The instrument utilizes cutting-edge technology to capture detailed images of cellular structures and processes with exceptional clarity and precision.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Skov3 cells, by American Type Culture Collection (1 mentions)
A2780cr, by Merck Group (1 mentions)
A2780 cells, by Merck Group (1 mentions)
Si hottip, by GenePharma (1 mentions)
Si zeb1, by GenePharma (1 mentions)
Siube2c, by GenePharma (1 mentions)
3 protocols using sizeb2
1
Ovarian Cancer Cell Line Comparison
2
Cell Transfection with UBE2C, ZEB1, and ZEB2
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For cell transfection, recombinant pcDNA3.1 plasmid contained UBE2C, ZEB1 or ZEB2, UBE2C small interfere RNA (siUBE2C, GenePharma Co. Ltd., Shanghai, China), ZEB1 small interfere RNA (siZEB1, GenePharma Co. Ltd., Shanghai, China), ZEB2 small interfere RNA (siZEB2, GenePharma Co. Ltd., Shanghai, China), and negative control oligonucleotides (NCO, GenePharma Co. Ltd.) were introduced into the A549, H1299, A549/DDP and H1299/DDP cells by using lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. The selected sequences for knockdown as follows:
3
miR-212-3p Modulates ZEB2 Expression
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miR-212-3p mimic (miR-212-3p; 5′-UAACAGUCUCCAGUCACGGCC-3′), mimic negative control (miR-NC; the non-targeting sequence, 5′-CGAUCGCAUCAGCAUCGAUUGC-3′), miR-212-3p inhibitor (5′-GGCCGUGACUGGAGACUGUUA-3′), inhibitor negative control (miR-NC inhibitor; the non-targeting sequence, 5′-CUAACGCAUGCACAGUCGUACG-3′), pcDNA3.1-ZEB2 overexpression vector (ZEB2), pcDNA3.1 vector (Thermo Fisher Scientific, Inc.) alone as overexpression vector negative control (vector), small interfering (si)RNA against ZEB2 (siZEB2; 5′-UGAUAUUGUUUCUCAUUCGGC-3′), and siRNA negative control (scrambled; 5′-UUCUCCGAACGUGUCACGUTT-3′) were obtained from Shanghai GenePharma Co., Ltd. Transient transfection of Huh7 and Huh7/PTX cells with 40 nM oligonucleotides or vector was performed by using Lipofectamine™ 2000 (Invitrogen; Thermo Fisher Scientific, Inc.), according to the manufacturer's instructions. Cells were collected for further analyses after 48 h post-transfection.
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