Hnrnpa3

Native RNA immunoprecipitation (RIP) was performed using published protocol.^{36 (link)} Briefly, nuclear lysates from RAW cells were diluted in RIP buffer and incubated with indicated antibodies at manufacturer-recommended dilutions. RNA-protein complexes were captured on magnetic-IgG beads, and bound RNA was isolated and analyzed by RT-qPCR. Antibodies used were PARP1 (1:100), HNRNPA3 (1:1000), SUPT16H (1:500, Proteintech, Cat number 20551-1-AP), SMARCA5 (1:500, Proteintech, Cat number 13066-1-AP), and Rabbit IgG control (1:1000). Cross-linked RIP was performed following published protocol.^{37 (link)}

The following commercial primary antibodies were used: hnRNPA3 (rabbit polyclonal, 25142–1-AP, Proteintech); CPSF6 (rabbit polyclonal, A301-356A, Bethyl); NCBP1 (rabbit polyclonal, 10349–1-AP, Proteintech); DAZAP1 (rabbit polyclonal, A303-984A, Bethyl); EXOSC3 (rabbit polyclonal, 15062–1-AP, Proteintech); SAFB (rabbit polyclonal, 21857–1-AP, Proteintech); GFP (mouse monoclonal, sc-9996, Santa Cruz); Flag (DYKDDDDK Tag, mouse monoclonal, 9A3, Cell Signaling); beta-actin (mouse monoclonal, A5441, Sigma). Antibodies were used at 1:1,000 dilution for immunostaining and western blot.