Cells were placed on glass coverslips in 12-well plates and fixed with absolute methanol at −20 °C for 5 min. Cells were blocked with 0.5% (v/v) Triton X-100 in PBS and 3% (w/v) bovine serum albumin (BSA) and incubated with anti-Acetyl-Histone H3 (Lys9) (Cell Signaling, Danvers, MA, USA), anti-CK7 (BD Biosciences, San Jose, CA, USA), and anti-CK14 (Cell Signaling, Danvers, MA, USA) as indicated. Cells were then washed three times and incubated with FITC or TRITC-conjugated secondary antibody for 60 min at RT and then stained with Hoechst 33,342 for visualization of DNA content. Images were taken using a QImaging ExiAqua monochrome digital camera attached to a Nikon Eclipse 80i Microscope (Nikon, Melville, NY) and visualized with QCapturePro software.
Anti ck7
Manufactured by BD
Sourced in United States
Anti-CK7 is a laboratory reagent used for the detection and identification of cytokeratin 7 (CK7) in biological samples. CK7 is a protein commonly expressed in various epithelial cell types, and its detection can be useful in certain diagnostic and research applications.
Automatically generated - may contain errors
2 protocols using anti ck7
1
Immunofluorescence Staining of Histone Modifications
2
Immunofluorescence Staining of Histone Modifications
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Cells were placed on glass coverslips in 12-well plates and fixed with absolute methanol at −20 °C for 5 min. Cells were blocked with 0.5% (v/v) Triton X-100 in PBS and 3% (w/v) bovine serum albumin (BSA) and incubated with anti-Acetyl-Histone H3 (Lys9) (Cell Signaling, Danvers, MA, USA), anti-CK7 (BD Biosciences, San Jose, CA, USA), and anti-CK14 (Cell Signaling, Danvers, MA, USA) as indicated. Cells were then washed three times and incubated with FITC or TRITC-conjugated secondary antibody for 60 min at RT and then stained with Hoechst 33,342 for visualization of DNA content. Images were taken using a QImaging ExiAqua monochrome digital camera attached to a Nikon Eclipse 80i Microscope (Nikon, Melville, NY) and visualized with QCapturePro software.
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