Cell stimulating antibodies to CD3 (clone OKT-3), CD28 (clone AF-342-PB) and CD46 (clone 344519) were all purchased from R&D Systems. Neutralizing antibodies to IL-4 (clone 8D4-8) and IL-10 (clone JES3-19F1) were both from BD Pharmingen. Antibodies used in flow cytometric analysis were FITC-labeled anti-CD46 (clone MEM-258), BV421-labeled anti-CD122 (clone TU27), PE-labeled anti-CD132 (clone AG184) from BioLegend, and FITC-labeled anti-CD19 (clone 4G7-2E3) and APC-labeled anti-CD138 (clone 359103), both from R&D Systems, while the APC-labelled anti-CD25 (clone M-A251) was purchased from BD Pharmingen. Recombinant human IL-2 and IL-10 were purchased from Pepro-Tech and soluble CD46 was prepared as previously described.10 The MMP-9 inhibitor was purchased from Calbiochem (CAS 1177749-58-4) and used at the concentrations indicated in the respective experiments.
Recombinant human il 2 and il 10
Manufactured by Thermo Fisher Scientific
Recombinant human IL-2 and IL-10 are cytokine proteins produced through recombinant DNA technology. IL-2 is involved in the activation and proliferation of T cells, while IL-10 is an anti-inflammatory cytokine that regulates the immune response.
Automatically generated - may contain errors
Lab products found in correlation
Cd46 clone 344519, by R&D Systems (2 mentions)
Il 4 clone 8d4 8, by BD (2 mentions)
Il 10 clone jes3 19f1, by BD (2 mentions)
Fitc labeled anti cd19 clone 4g7 2e3, by R&D Systems (2 mentions)
Apc labeled anti cd138 clone 359103, by R&D Systems (2 mentions)
Apc labelled anti cd25 clone m a251, by BD (2 mentions)
2 protocols using recombinant human il 2 and il 10
1
Stimulating T and B Cell Responses
2
Immune Cell Activation and Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell stimulating antibodies to CD3 (clone OKT‐3), CD28 (clone AF‐342‐PB) and CD46 (clone 344519) were all purchased from R&D Systems. Neutralizing antibodies to IL‐4 (clone 8D4‐8) and IL‐10 (clone JES3‐19F1) were both from BD Pharmingen. Antibodies used in flow cytometric analysis were FITC‐labeled anti‐CD46 (clone MEM‐258), BV421‐labeled anti‐CD122 (clone TU27), PE‐labeled anti‐CD132 (clone AG184) from BioLegend, and FITC‐labeled anti‐CD19 (clone 4G7‐2E3) and APC‐labeled anti‐CD138 (clone 359103), both from R&D Systems, while the APC‐labelled anti‐CD25 (clone M‐A251) was purchased from BD Pharmingen. Recombinant human IL‐2 and IL‐10 were purchased from PeproTech and soluble CD46 was prepared as previously described.10 The MMP‐9 inhibitor was purchased from Calbiochem (CAS 1177749‐58‐4) and used at the concentrations indicated in the respective experiments.
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