The ABX Pentra 400 analyzer (Horiba Ltd, Kyoto, Japan) is a compact bench top clinical biochemistry analyzer [20] (link). The configuration of the open channel was based on the Roche Total Mycophenolic Acid® assay package insert. Briefly, the analyzer added 3 µL of sample to 180 µL of R1 (cycle 1; a cycle every 12 s). After mixing and incubation for 5 min, 19 µL of R2 was added and mixed (cycle 26). At this point, bichromatic measurements (340/405 nm) were performed. The slope of the reaction rate is determined between cycles 49 and 62. The measuring temperature (37 °C) was controlled by an air bath. The assay was calibrated with 6-point calibration at 0, 1, 3, 5, 10 and 15 mg/L (Roche Total MPA Calibrators; Roche Diagnostics) using a logit/log4 calculation mode. All samples above 15 mg/L were manually diluted (1:3) with the diluent (equivalent to the 0 mg/L calibrator) from the Roche Total MPA Calibrators.
The assay was bidirectionally interfaced to the Laboratory Information System (LIS) using barcoded tubes. The reagents were stored in the integrated refrigerated reagent compartment (2–10 °C).
The assay was bidirectionally interfaced to the Laboratory Information System (LIS) using barcoded tubes. The reagents were stored in the integrated refrigerated reagent compartment (2–10 °C).