Sc 58125

Celecoxib, NS-398, SC-58125, SC-236, nimesulide, and SC-560 were purchased from Cayman Chemical (Ann Arbor, MI, USA). GCDCA, dimethyl sulfoxide (DMSO), L-NAME, MDL-12330A, and indomethacin were from Sigma-Aldrich (St. Louis, MO, USA). COX-1 siRNA, COX-2 siRNA, and scrambled siRNA were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Monoclonal antibody (MAb) against mouse COX-1 and polyclonal antibody against rabbit COX-2 were obtained from Abcam (Cambridge, MA, USA). Mouse MAb against HA tag was purchased from OriGene (Rockville, MD, USA). Synthetic PGE₂ was purchased from Tocris Bioscience (Ellisville, MO, USA). The anti-PSaV capsid MAb and the anti-PSaV VPg polyclonal antibody were previously described (41 (link)). The secondary antibodies used were horseradish peroxidase-conjugated goat immunoglobulin against rabbit IgG (Cell Signaling, Beverly, MA, USA) and mouse IgG (Santa Cruz) and fluorescein isothiocyanate (FITC)-conjugated goat immunoglobulin against rabbit IgG (Jackson Immuno Research Laboratory, West Grove, PA, USA).

We greased fly medium by paste of hydrolyzed yeast containing one of the substances. Control untreated animals were fed by yeast past without substances. To make the hydrolyz at yeast were boiled in water bath for 30 minutes. To prepare the 100 ml of paste 50 g of dry yeast per 60 mL of water were used.
Flies were treated throughout their whole lives with the substances as follows: aspirin (2-(acetyloxy)-benzoic acid), valeryl salicylate (2-[(1-oxopentyl)oxy]-benzoicacid), trans-resveratrol ((E)-5-[2-(4-hydroxyphenyl)ethenyl]-1,3-benzenediol), SC-560 (5-(4-chlorophenyl)-1-(4-methoxyphenyl)-3-(3fluoromethyl)-1H-pyrazole), APHS (2-(2-heptynylthio)-phenol-acetate), NS-398 (N-[2-(cyclohexyloxy)-4-nitrophenyl]-methanesolfonamide), SC-58125 (5-(4-fluorophenyl)-1-[4-(methylsulfonyl)-phenyl]-3-(trifluoromethyl)-1H-pirazole), valdecoxib (4-(5-methyl-3-phenyl-4-isoxazolyl)-benzenesulfonamide), CAY10404 (3-(4-methylsulphonylphenyl)-4-phenyl-5-trifluoromethylisoxazole), licofelone (6-(4-chlorophenyl)-2, 3-dihydro-2,2-dimethyl-7-phenyl-1H-pyrrolizine-5-acetic acid) at the concentrations of 0.05, 0.5, 1 μM (Cayman Chemical, USA).