Cy3 conjugated goat anti rat antibody

Manufactured by Jackson ImmunoResearch

Sourced in United Kingdom

Cy3-conjugated goat anti-rat antibody is a secondary antibody that is labeled with the fluorescent dye Cy3. This antibody is designed to detect and visualize the presence of rat target proteins in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Mouse anti neun antibody, by Merck Group (4 mentions) Rat anti brdu antibody, by Agilent Technologies (4 mentions) Vectashield mounting medium, by Vector Laboratories (4 mentions) Fitc conjugated anti mouse antibody, by Jackson ImmunoResearch (3 mentions) Axiovert 200 microscope, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Fluorescence mounting medium, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Goat anti-rat Cy3 Cy3 anti-rat antibody Anti-rat Cy3 Cy3 anti-goat

5 protocols using cy3 conjugated goat anti rat antibody

Quantifying Neurogenesis and Angiogenesis Post-Injury

Check if the same lab product or an alternative is used in the 5 most similar protocols

Double immunostaining was performed to identify newly generated endothelial cells (BrdU/EBA+) and newly formed mature neurons (BrdU/NeuN+) 35 days after injury. Briefly, after being deparaffinized and rehydrated, brain sections were boiled in 10 mM citric acid buffer (pH 6) for 10 minutes. After washing with PBS, sections were incubated in 2.4 N HCl at 37°C for 20 minutes. Sections were incubated with 1% bovine serum albumin containing 0.3% Triton X-100 in PBS. Sections were then incubated with mouse anti-NeuN antibody (1:200; Chemicon) or anti-EBA at 4°C overnight. For negative controls, primary antibodies were omitted. Fluorescein isothiocyanate–conjugated anti–mouse antibody (1:400; Jackson ImmunoResearch) was added to sections at room temperature for 2 hours. Sections were then incubated with rat anti-BrdU antibody (1:200; Dako) at 4°C overnight, and subsequently incubated with Cy3-conjugated goat anti–rat antibody (1:400; Jackson ImmunoResearch) at room temperature for 2 hours. Each of the steps was followed by three 5-minute rinses in PBS. Tissue sections were mounted with Vectashield mounting medium (Vector Laboratories).

Zhang Y., Zhang Z.G., Chopp M., Meng Y., Zhang L., Mahmood A, & Xiong Y. (2016). Treatment of traumatic brain injury in rats with N-acetyl-seryl-aspartyl-lysyl-proline. Journal of neurosurgery, 126(3), 782-795.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Post-TBI Neurogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Newly generated endothelial cells and newborn mature neurons in the lesion boundary zone and dentate gyrus 35 days after TBI were identified by double labeling for BrdU with EBA or NeuN, respectively. Briefly, after being deparaffinized and rehydrated, brain sections were boiled in 10 mM citric acid buffer (pH 6) for 10 minutes. After washing with PBS, sections were incubated in 2.4 N HCl at 37°C for 20 minutes. Sections were incubated with 1% BSA containing 0.3% Triton-X-100 in PBS. Sections were then incubated with mouse anti-NeuN antibody (1:200; Chemicon, Temecula, CA) or anti-EBA at 4°C overnight. For negative controls, primary antibodies were omitted. FITC-conjugated anti-mouse antibody (1:400; Jackson ImmunoResearch, West Grove, PA) was added to sections at room temperature for 2 hours. Sections were then incubated with rat anti-BrdU antibody (1:200; Dako, Glostrup, Denmark) at 4°C overnight. Sections were then incubated with Cy3-conjugated goat anti-rat antibody (1:400; Jackson ImmunoResearch, West Grove, PA) at room temperature for 2 hours. Each of the steps was followed by three 5-minute rinses in PBS. Tissue sections were mounted with Vectashield mounting medium (Vector laboratories, Burlingame, CA).

Zhang Y., Chopp M., Meng Y., Katakowski M., Xin H., Mahmood A, & Xiong Y. (2015). Effect of exosomes derived from multipluripotent mesenchymal stromal cells on functional recovery and neurovascular plasticity in rats after traumatic brain injury. Journal of neurosurgery, 122(4), 856-867.

+ Open protocol

+ Expand

Immunofluorescence Staining of α-Tubulin in WE-68 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

WE-68 cells were seeded on collagen-coated cover slips. 24 h after treatment, cells were fixed in 1 ml methanol for 5 min at − 20 °C and washed three times in PBS. Prior to staining, each sample was blocked in 1% BSA in PBS for 20 min at room temperature. Cells were incubated with a rat anti-α-tubulin antibody (Bio-Rad, #MCA77G; 1:500) for 1 h at room temperature. Cover slips were washed three times with PBS, followed by incubation with a secondary Cy3-conjugated goat anti-rat antibody (Jackson ImmunoResearch, Ely, UK, #112–165-167; 1:1000) for 45 min at room temperature in the dark. Finally, cover slips were washed three times with PBS and stained with 5 µg/ml DAPI (Sigma) for 20 min at room temperature, followed by repeated washing with PBS and sealing with Fluorescence Mounting Medium (Agilent, Waldbronn, Germany). Mounted cells were imaged using a Zeiss (Jena, Germany) Axiovert 200 microscope.

Kerschner-Morales S.L., Kühne M., Becker S., Beck J.F, & Sonnemann J. (2020). Anticancer effects of the PLK4 inhibitors CFI-400945 and centrinone in Ewing’s sarcoma cells. Journal of Cancer Research and Clinical Oncology, 146(11), 2871-2883.

+ Open protocol

+ Expand

Identifying Neurogenesis after Traumatic Brain Injury

Check if the same lab product or an alternative is used in the 5 most similar protocols

Newly generated endothelial cells and newborn mature neurons in the lesion boundary zone and dentate gyrus 35 days after TBI were identified by double labeling for BrdU with EBA or neuronal nuclei (NeuN), respectively. In brief, after being deparaffinized and rehydrated, brain sections were boiled in 10 mM citric acid buffer (pH 6) for 10 minutes. After washing with PBS, sections were incubated in 2.4 N HCl at 37°C for 20 minutes. Sections were incubated with 1% BSA containing 0.3% Triton-X-100 in PBS. Sections were then incubated with mouse anti-NeuN antibody (1:200; Chemicon, Temecula, CA) or anti-EBA at 4°C overnight. For negative controls, primary antibodies were omitted. FITC-conjugated anti-mouse antibody (1:400; Jackson ImmunoResearch, West Grove, PA) was added to sections at room temperature for 2 hours. Sections were then incubated with rat anti-BrdU antibody (1:200; Dako, Glostrup, Denmark) at 4°C overnight. Sections were then incubated with Cy3-conjugated goat anti-rat antibody (1:400; Jackson ImmunoResearch, West Grove, PA) at room temperature for 2 hours. Each of the steps was followed by three 5-minute rinses in PBS. Tissue sections were mounted with Vectashield mounting medium (Vector laboratories, Burlingame, CA).

Zhang Y., Chopp M., Zhang Z.G., Katakowski M., Xin H., Qu C., Ali M., Mahmood A, & Xiong Y. (2016). Systemic administration of cell-free exosomes generated by human bone marrow derived mesenchymal stem cells cultured under 2D and 3D conditions improves functional recovery in rats after traumatic brain injury. Neurochemistry international, 111, 69-81.

+ Open protocol

+ Expand

Dual Labeling of Newborn Neurons in DG

Check if the same lab product or an alternative is used in the 5 most similar protocols

Double fluorescent staining with NeuN and BrdU was performed to identify newborn mature neurons in the DG. Briefly, after being deparaffinized and rehydrated, brain sections were boiled in 10 mM citric acid buffer (pH 6) for 10 minutes. After washing with PBS, sections were incubated in 2.4 N HCl at 37°C for 20 minutes. Sections were incubated with 1% BSA containing 0.3% Triton-X-100 in PBS. Sections were then incubated with mouse anti-NeuN antibody (1:200; Chemicon) at 4°C overnight. For negative controls, primary antibodies were omitted. FITC-conjugated antimouse antibody (1:400; Jackson ImmunoResearch, West Grove, PA) was added to sections at room temperature for 2 hours. Sections were then incubated with rat anti-BrdU antibody (1:200; Dako) at 4°C overnight. Sections were then incubated with Cy3-conjugated goat antirat antibody (1:400; Jackson ImmunoResearch) at room temperature for 2 hours. Each of the steps was followed by three 5-minute rinses in PBS. Tissue sections were mounted with Vectashield mounting medium (Vector laboratories).

Zhang Y., Chopp M., Zhang Z.G., Zhang Y., Zhang L., Lu M., Zhang T., Winter S., Doppler E., Brandstäetter H., Mahmood A, & Xiong Y. (2019). Cerebrolysin Reduces Astrogliosis and Axonal Injury and Enhances Neurogenesis in Rats After Closed Head Injury. Neurorehabilitation and neural repair, 33(1).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!