The largest database of trusted experimental protocols

4 protocols using protocatechuic acid

1

HPLC-DAD Analysis of Phytochemicals

Check if the same lab product or an alternative is used in the 5 most similar protocols
Solvents for the HPLC-DAD analyses were of analytical grade and were purchased from Sigma (St. Louis, MO, USA). Water was purified by a Milli-Q Plus system from Millipore (Milford, MA, USA). The following commercial compounds were used as standards: Gallic acid, pyrogallol, 4-hydroxy benzhydrazide, epigallocatechin, vanillic acid, (+)-catechin hydrate, syringic acid, phloroglucinol, p-anisic acid, dexamethasone, chlorogenic acid, caffeic acid, p-coumaric acid, trans-ferulic acid, naringin, 2-amino-3,4-dimethyl benzoic acid, coumarin, morin hydrate, luteolin, hesperetin, alizarin, biotin, trans-chalcone, rutin hydrate, myricetin, quercetin, rhein, 3-hydroxyflavone, and emodin were purchased from Sigma–Aldrich Co. Catechin gallate and protocatechuic acid were obtained from Santa Cruz Biotech (Santa Cruz, CA, USA). All other chemicals were analytical grade.
+ Open protocol
+ Expand
2

Optimizing Fluorescence Imaging Conditions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fluorescence imaging experiments were in 80-500 mM NaCl as noted, 20 mM HEPES (pH 7.4), 0.02% Tween 20, 0.1 mM D-biotin (Invitrogen, Carlsbad, CA, USA), 0.5 mg ml−1 BSA together with 2.2mM protocatechuic acid (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and 37nM protocatechuate-3,4-dioxygenase (Sigma-Aldrich) as oxygen scavengers[58 (link)]. 20 mM Bis-Tris (pH 6.2), 20 mM Tris (pH 8.6) or 20 mM CHES (pH 9.8) was used instead of 20 mM HEPES (pH 7.4) when noted. 20 mM HEPES (pH 7.4), 20 mM Bis-Tris (pH 6.2), 20 mM Tris (pH 8.6) or 20 mM CHES (pH 9.8) contributed 0.008, 0.014, 0.005 or 0.015 M to ionic strength[59 (link)]. For fine-tuning the driving pressure of the flow system in experiments that included measurements below 80 dyn cm−2, 2.5 × 10–6 w/v 1 μm diameter polystyrene particles (PP-10-10, Spherotech, Lake Forest, IL, USA) were added as flow tracers. Some VWF multimers tethered to the surface appear to become more compact after being stretched repeatedly. The fraction of VWF molecules becoming compact increases at extremely high salt concentrations and extreme pH levels. This compaction is perhaps due to the formation of extra attachments between VWF and the surface near the tether point.
+ Open protocol
+ Expand
3

Antioxidant Assays and Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dimethyl sulfoxide, diphenylamine, 5,5′‐dithiobis(2‐nitrobenzoic acid), epinephrine, guanidine hydrochloride, hydrogen peroxide, N‐ethylmaleimide, sodium chloride, thiobarbituric acid were procured from Sigma‐Aldrich (St. Louis, MO). Nitroblue tetrazolium, 2,2′‐dipyridyl, l‐glutathione (reduced), and protocatechuic acid are products of Santa Cruz Biotechnology. All other reagents are products of Sigma‐Aldrich (St. Louis, MO).
+ Open protocol
+ Expand
4

Exploring Cyclophosphamide and Protocatechuic Acid

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cyclophosphamide and protocatechuic acid were purchased from (Santa Cruz, CA, USA). Glutathione (GSH), malondialdehyde (MDA), nitric oxide (NO), and catalase colorimetric kits were determined using Biodiagnostic kit, Cairo, Egypt. Sirtuin 1 (SIRT1) was determined using ELISA kits procured from (SunRed Biotech Co., Ltd, China). Tumor necrosis factor alpha (TNF-α), Interleukin-17 (IL-17), nuclear factor kappa B (NF-κB), inhibitor of nuclear factor kappa beta kinase subunit beta (IKBKB), proxisome proliferator-activated receptor gamma (PPARγ), cyclooxegnase-2 (COX-2), protein kinase C (PKC), and myeloperoxidase (MPO) were determined using ELISA kits procured from Sunlong Biotech Co., Ltd, China.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!