Cells were placed on slides and fixed with 4% paraformaldehyde for 15 min, incubated with 0.3% triton solution for 10 min, sealed with 5% BSA for 30 min and then incubated with Ki67 (1:500; CST, United States) primary antibody at 4°C overnight. The following morning, the cells were incubated with a fluorescence secondary antibody (1:300, Abcam, United States) at room temperature for 1 h. Images were then acquired by a confocal microscope.
Fluorescence secondary antibody
Manufactured by Abcam
Sourced in United States, United Kingdom
Fluorescence secondary antibody is a laboratory reagent used to detect and visualize target proteins in various experimental techniques. It is designed to bind to a primary antibody that has already recognized and attached to the protein of interest, allowing for fluorescent detection and quantification.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fluorescence secondary antibody
1
Immunocytochemical Analysis of Ki67
2
Immunofluorescence Staining of Dendritic Cells
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The DCs were first seeded on a cover slide in a 6-well plate, and then fixed with 4% paraformaldehyde permeated with 0.5% Triton X-100. After washing, the DCs were sealed with 1% bovine serum albumin (BSA), anti-CD11c (Abcam, Cambridge, UK) at 4 °C overnight, and then incubated with fluorescence secondary antibody (Abcam, Cambridge, UK) for 1 h. Then, 4',6-diamidino-2-phenylindole-2HCl was dripped onto the DCs for 5 min, and images were taken under a fluorescence microscope.
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