Nucleic acid solution was prepared by dissolving 10 mg of commercially available deoxyribonucleic acid (double-stranded (ds) molecule), DNA, fish sperm from salmon testes (Sigma-Aldrich) in 10 mL of doubly distilled water. 24 h or more were needed for the dissolution at 4 °C, accompanied by occasional gentle shaking.
Salmon testes
Manufactured by Merck Group
Sourced in United States
Salmon testes are a type of lab equipment used for various scientific research and experiments. They serve as a source of biological material, specifically male reproductive organs, derived from salmon. The core function of salmon testes is to provide a natural, animal-derived sample for researchers to study and analyze.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using salmon testes
1
Preparation of DNA Solution
2
Yeast Transformation by Lithium Acetate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Yeast cells were transformed by the lithium acetate method with 1 µg plasmid or 5 µg DNA fragment, respectively (Gietz et al. 1995 (link)). In addition, 50 µg of carrier DNA, deoxyribonucleic acid sodium salt from salmon testes (Sigma Aldrich, USA), was added to enhance the transformation efficiency (Gietz et al. 1995 (link)).
3
Quantifying DNase Activity via SRED Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We quantified DNase activity by the SRED assay [27] . This involved agarose gels containing fluorescent doublestranded DNA being prepared by dissolving 0.13 mg mL À1 DNA from salmon testes (Sigma-Aldrich) in buffer with 100 mM MES pH 6.5, 20 mM MgCl 2 , 2 mM CaCl 2 and 29 SYBR Safe (Life Technologies). The DNA solution was heated at 50 °C for 10 min and mixed with an equal volume of 2% agarose GP-36 (Gerbu, Heidelberg, Germany). The mixture was poured into trays and stored at RT until solidification. Two microliters of sample were applied to wells of 1.0-mm radius. After 24 h incubation at 37 °C in a humid chamber, the fluorescence of the gels was recorded with a fluorescence scanner (Molecular Imager FX; Bio-Rad, Munich, Germany). Image J (NIH) was used for the quantification of signal intensity and the radius of the circles reflecting DNase activity.
4
Viscosity Changes in DNA-Drug Interactions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fifteen mL dsDNA (deoxyribonucleic acid sodium salt from Salmon Testes, Sigma-Aldrich, D1626-1G) solution was prepared at 1 × 10 -3 M in 80 mM HEPES buffer for each working sample.
Stock solutions prepared in DMSO were added according to the gradual increasing [drug]/[DNA] (r) ratios of 0.025, 0.05, 0.075, 0.1, 0.125, 0.15, 0.175 and 0.2. Viscosity values, η, (unit: cP) were directly obtained by running 0# spindle in working samples at 60 rpm via DV-II-programmable digital viscometer equipped with enhanced brookfield UL Adapter at room temperature. Data were presented as η/η o versus [compound]/[DNA] ratio, in which η o and η refers to viscosity of each DNA working sample in the absence and presence of complex.
Stock solutions prepared in DMSO were added according to the gradual increasing [drug]/[DNA] (r) ratios of 0.025, 0.05, 0.075, 0.1, 0.125, 0.15, 0.175 and 0.2. Viscosity values, η, (unit: cP) were directly obtained by running 0# spindle in working samples at 60 rpm via DV-II-programmable digital viscometer equipped with enhanced brookfield UL Adapter at room temperature. Data were presented as η/η o versus [compound]/[DNA] ratio, in which η o and η refers to viscosity of each DNA working sample in the absence and presence of complex.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!