Mouse mast cell protease 1 mmcp 1

Galactose was purchased from Macklin (Shanghai, China). Rabbit anti-crab TM IgG pAb was prepared in our laboratory previously and goat anti-rabbit IgG-HRP antibody was from Abmart (Berkeley Heights, NJ, USA). Granulocyte macrophage colony stimulating factor (GM-CSF), IL-4 were obtained from Sigma-Aldrich (St. Louis, MO, USA). RPMI 1640 and fetal bovine sera (FBS) were purchased from Invitrogen (New York, NY, USA). CD11c for flow cytometry analysis were purchased from Biolegend (San Diego, CA, USA). Imject Alum was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Goat anti-mouse IgE, IgG1, and IgG2a antibodies were purchased from Abcam (Cambridge, MA, USA). The ELISA kits of IL-4, IL-10, IL-13, interferon (IFN)-γ, mouse mast-cell protease-1 (mMCP-1) and transforming growth factor-β (TGF-β) were purchased from R&D Systems (Minneapoils, MN, USA). An ELISA kit of histamine was purchased from IBL (Hamburg, Germany).