The mouse macrophage cell line RAW264.7 was purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). High-glucose DMEM medium (11965092) and fetal bovine serum (10100147) were purchased from Gibco (Grand Island, USA). A total RNA extraction kit (BSC52M1) was obtained from Bori Biotech Co., Ltd. (Anhui, China). A reverse transcription kit (RR047A) and fluorescent quantitative PCR reagent (RR420A) were purchased from Takara (Kyoto, Japan). A cell Counting Kit (CCK-8, FC101-02) was obtained from TransGen Biotech Co., Ltd. (Beijing, China). CD80 (11-0801-82), CD86 (25-0862-82) were purchased from Thermo Fisher (MA, USA). Tetramethylrhodamine isothiocyanate (TRITC Dextran, T1162) was purchased from Merck (NY, USA). Azoxymethane (AOM, A5486) was purchased from Sigma (MO, USA). Dextran sulfate sodium (DSS, 216011090) was obtained from MP Biomedicals (CA, USA). An immunohistochemical antibody kit (97624T) was obtained from Cell Signaling Technology (MA, USA). TAK-242 (A3850), PDTC (B6422), KG-501 (B8380), SR11302 (A8185), and LY294002 (A8250) were purchased from APExBIO (Houston, USA).
Ly294002 a8250
Manufactured by Apexbio
Sourced in United States
LY294002 (A8250) is a small molecule that inhibits the PI3 kinase pathway. It is commonly used in research applications as a tool compound to study signal transduction and cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
High glucose dmem medium 11965092, by Thermo Fisher Scientific (1 mentions)
Raw264, by National Collection of Authenticated Cell Cultures (1 mentions)
Ab179463, by Abcam (1 mentions)
Ab131339, by Abcam (1 mentions)
Ab76431, by Abcam (1 mentions)
Ab178846, by Abcam (1 mentions)
Ab192623, by Abcam (1 mentions)
Ab170941, by Abcam (1 mentions)
P pi3k antibody, by Cell Signaling Technology (1 mentions)
Anti β actin sc 47778, by Santa Cruz Biotechnology (1 mentions)
2 protocols using ly294002 a8250
1
Macrophage RAW264.7 cell culture protocol
2
Fluoxetine and Ginsenoside Modulation of LPS-Induced Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Fluoxetine hydrochloride capsule (Flu) was purchased from Patheon France and was packaged by Lilly Suzhou Pharmaceutical Co., Ltd. Ginsenosides Rg1 (GRg1, B21057), and saikosaponin d (Ssd, B20150) were purchased from Yuanye Biotech (Shanghai, China). Lipopolysaccharide (LPS: L3129) was purchased from Sigma–Aldrich. A small-molecule-specific inhibitor of TLR4 signaling (TAK242, A3850) and PI3K/AKT inhibitor (LY294002, A8250) were from Apex Bio (Houston, Texas). TNF-α, IL-6, and IL-1β ELISA kits were purchased from BioProducts MD (Middletown, MD, USA). The antibodies to PI3K (ab178846), p-AKT (ab192623), AKT (ab179463), p-PTEN (ab76431), PTEN (ab170941), p-FOXO1 (ab131339), and FOXO1 (ab52857) were from Abcam, TLR4 antibody (AF7017) was from Affinity Biosciences, the p-PI3K-antibody (#4228) was from Cell signaling, the anti-β-actin (sc-47778) and goat anti-rabbit IgG (sc-516102) were from Santa Cruz Biotechnology.
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