All blood samples were analyzed at the Department of Clinical Biochemistry at Herlev Hospital, except plasma 1,25-(OH)2 vitamin D, and FGF23. Plasma phosphate was analyzed in ethylenediamine tetraacetic acid (EDTA) with standard laboratory methods on a Vitros analyzer (Ortho-Clinical Diagnostic, Raritan, NJ), and PTH was analyzed using the ADVIA Centaur intact PTH assay (Siemens Healthineers, Erlangen, Germany). FGF23 was determined in EDTA plasma using a chemiluminescence immunoassay assay (CLIA) and 1,25-(OH)2 vitamin D was measured in EDTA plasma using CLIA. Both assays were performed on the automated analyzer Liaison XL (Diasorin, Saluggia, Italy). Intermediary precision for the 2 assays was <10%.
Advia centaur intact pth assay
Manufactured by Siemens
Sourced in Germany, United States
The ADVIA Centaur intact PTH assay is a laboratory diagnostic test used to measure the concentration of intact parathyroid hormone (PTH) in human blood samples. The assay is designed to provide accurate and reliable results for the evaluation of PTH levels, which can be used in the assessment of various medical conditions.
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2 protocols using advia centaur intact pth assay
1
Biochemical Markers in Vitamin D Metabolism
2
Hormonal and Bone Metabolism Markers
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Body mass index was calculated as weight (kg)/[height (m)] 2 . Serum levels of leptin, adiponectin, resistin, FGF23, a-Klotho, IGF1, GH, and osteocalcin were quantified using commercially available ELISA Research kits (Elabscience Biotechnology, USA). Serum parathyroid hormone (PTH) was quantified by electrochemiluminescence (ECLIA), using commercial kits (Advia Centaur Intact PTH Assay, Siemens Healthcare Diagnostics Inc., USA). Free T4, cortisol, oestradiol (in women), testosterone (in men), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) were routinely measured in the Acro group via ECLIA (Immulite 2000 Immunoassay System, Siemens). Serum concentrations of calcium, phosphate, and glucose were determined by colorimetry (Cobas 6000 analyser, Roche). HbA1c was assessed via the ion-exchange high-performance liquid chromatography (HPLC) method. BMD at the lumbar spine (the mean BMD value for L1-L4 lumbar vertebrae), femoral neck, 1/3 radius, and whole-body levels were measured via DXA (Hologic Delphi A; Hologic Inc., USA) by 2 ho-
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