Cpi444

Graphite flakes (Alfa Aesar, 99.8%, natural graphite 325 mesh), 6-arm PEG-amine (JenKem Technology, 15 kDa), sulfuric acid (H₂SO₄, Rankem, 95%−98%), potassium permanganate (KMnO₄, Aldrich, 99%), phosphoric acid (H₃PO₄), hydrogen peroxide (30% w/v) and potassium hydroxide (KOH, powder) from Finar Scientific, Vatalanib (cat#A3969, APExBIO), CPI444 (cat#1202402-40-1, MedChemExpress), and GBM U87 cell lines were obtained from the National Centre for Cell Sciences (NCCS) Pune. The origin of GBM U87 (or U-87 MG) is a malignant glioma from a human male patient with epithelial morphology. It is from the primary brain tumor and grade IV glioma according to the WHO classification (29 (link)). MEM (Minimum Essential Medium Eagle, Cat#AL080A) media, trypsin-EDTA solution 1× 0.25% (Cat#TCL007), FBS (Fetal Bovine Serum, Cat#RM10681), antibiotic solution (penicillin and streptomycin), and PBS (Dulbecco’s Phosphate Buffered Saline, powder pH 7.4) were purchased from HiMEDIA, India. Fluorescein isothiocyanate (FITC, Invitrogen) and CCK-8 (Cell Counting Kit-8/Cytotoxicity assay kit) were purchased from Dojindo Molecular Technologies, USA.

NECA, adenosine and LPS were purchased from Sigma-Aldrich. Oxaliplatin, CPI-444, AZD4635 and AB928 were purchased from MedChemExpress, DZD2269 was designed and synthesized by Dizal Pharmaceuticals. All the compounds were dissolved in DMSO to prepare a 10 mM stock solution and stored in a nitrogen cabinet before use. Anti-PD-1 (BE0146) and Isotype control antibodies were purchased from Bioxcell. Anti-mouse CD45 PE (clone 30-F11), anti-mouse CD4 PerCP (clone RM4-5), anti-mouse CD8a FITC (clone 53 − 6.7), anti-CD45 FITC (clone HI30), anti-human CD8 PE (clone RPA-T8), anti-human CD4 FITC (clone PRA-T4), anti-CD14 PE (clone M5E2), anti-HLA-DR APC-H7 (clone G155-178), anti-CD83 APC (clone HB15e), anti-CD4 (clone, RPA-T4) and anti-CD8 (clone RPA-T8) were purchased from BD Bioscience. Anti-pCREB^Ser133 Alexa Fluor 647 (clone 87G3) and anti-mouse CD8 (clone D4W2Z) were purchased from Cell Signaling Technology. Anti-human CD3 antibody (clone OKT3) and anti-human CD28 antibody (clone CD28.2) were purchased from eBioscience. Anti-mouse CD3 antibody (clone 145-2C11) and anti-mouse CD28 antibody (clone E18) were purchased from BioLegend. Anti-mouse CD4 (EPR19514) was purchased from Abcam.