Ab261851

Manufactured by Abcam

Sourced in United States

Ab261851 is a mouse monoclonal antibody that specifically recognizes the human GAPDH protein. This antibody is suitable for use in Western blotting applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti laminin a c, by Santa Cruz Biotechnology (1 mentions) Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions) Anti flag, by Merck Group (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Passive lysis buffer (plb), by Promega (1 mentions) Ehigfbp3, by Thermo Fisher Scientific (1 mentions) Ab100647, by Abcam (1 mentions)

3 protocols using ab261851

Luciferase Assay and ELISA Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

To evaluate luciferase activity, cells were washed with PBS and lysed in 200 μL of Passive Lysis Buffer (E1941 Promega). Additionally, three cycles of freezing and thawing at −80°C and 37°C respectively were performed to enhance cell lysis. Finally, Renilla and Firefly luciferases were measured according to manufacturer’s instructions of the Dual Renilla Luciferase Reporter Assay (E1960 Promega) in a luminometer (Orion L Microplate Luminometer; Berthold Detection Systems).
For ELISA, cell supernatant was collected 48 h post transfection, centrifuged at 4,000 ×g for 10 min at 4°C, aliquoted, and stored at −20°C. Human CTGF ELISA was performed with a commercial kit (ab261851; Abcam) following manufacturer’s instructions. Antibodies used for western-blot⁴³ (link) were anti-FLAG (F3165; Sigma) diluted 1:1,000, anti-U1A (sc101149 from Santa Cruz and ab166890 from Abcam) diluted 1:1,000, anti-U170K (sc-9571; Santa Cruz) diluted 1:200, anti-U1C (sc-101549; Santa Cruz) diluted 1:200, anti-GAPDH (5174; Cell Signaling) diluted 1:10,000, anti-laminin a/c (sc-7292; Santa Cruz) diluted 1:1,000, and anti-HSP90 (13171-1-AP; Cell Signaling) diluted 1:1,000, and secondary antibodies used were anti-rabbit IgG HRP-linked antibody (#7074; Cell Signaling) diluted 1:10,000 and anti-mouse IgG HRP-linked antibody (A0168; Merck) diluted 1:20,000.

Rovira E., Moreno B., Razquin N., Hjerpe R., Gonzalez-Lopez M., Barrio R., Ruiz de los Mozos I., Ule J., Pastor F., Blazquez L, & Fortes P. (2022). U1A is a positive regulator of the expression of heterologous and cellular genes involved in cell proliferation and migration. Molecular Therapy. Nucleic Acids, 28, 831-846.

+ Open protocol

+ Expand

Serum IGFBP and CTGF Levels Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

A total of 4 mL venous blood was collected from each patient and HC in a blood collection tube with coagulating agent, and the samples were allowed to clot at room temperature for approximately 2 h. After clot formation, the samples were centrifuged at 2000g for 10 min. Then, the sera were collected and stored at −80°C.
Serum IGFBP and CTGF levels were detected using the following human ELISA kits: IGFBP-1 (ab213789, Abcam Cambridge, UK), IGFBP-2 (ab215082, Abcam), IGFBP-3 (EHIGFBP3, Thermo Fisher Scientific, Waltham, MA, USA), IGFBP-4 (ab230936, Abcam), IGFBP-6 (EHIGFBP6, Thermo Fisher Scientific), and CTGF (ab261851, Abcam).

Yang M., Chen Y., Yin G., Lin S., Liu H., Huang Y., Tian Y., Zhou Y., Luo F, & Xie Q. (2022). Elevated Serum IGFBP-2 and CTGF Levels Are Associated with Disease Activity in Patients with Dermatomyositis. Disease Markers, 2022, 9223883.

+ Open protocol

+ Expand

Circulating TGF-β1 and CTGF in Vascular Calcification

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

In this study, we used cryopreserved sera from community-dwelling older adults participating in a cytokine and miRNA study for measuring circulating TGF-β1 and CTGF, based on enzyme-linked immunosorbent assays (ELISAs) (ab100647 and ab261851; Abcam). Their VC severity was determined based on a validated classification scheme for AAC [45 (link)], which also demonstrated good applicability to our patients according to our prior work [13 (link), 46 (link)]. We identified those without AAC and those with severe AAC, and compared their circulating TGF-β1 and CTGF levels using the Student’s independent t-test, in order to validate the clinical implications of TGF-β1 and CTGF in VC.

Tsai Y.T., Yeh H.Y., Chao C.T., Huang J.W, & Chiang C.K. (2023). The context-dependent role of transforming growth factor-β/miR-378a-3p/connective tissue growth factor in vascular calcification: a translational study. Aging (Albany NY), 15(3), 830-845.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!